| 引用本文: | 孙清超,晋伟明,张力为,李少杰,李德生.食管鳞状细胞癌差异类泛素化调节[]因子的筛选及生物信息学分析[J].生物信息学,2026,24(1):32-43. |
| SUN Qingchao,JIN Weiming,ZHANG Liwei,LI Shaojie,LI Desheng.Screening and bioinformatics analysis of differential ubiquitinizationregulators in esophageal squamous cell carcinoma[J].Chinese Journal of Bioinformatics,2026,24(1):32-43. |
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| 食管鳞状细胞癌差异类泛素化调节[]因子的筛选及生物信息学分析 |
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孙清超1,2,晋伟明1,张力为1,李少杰1,李德生1,2
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(1.新疆医科大学第一附属医院 胸外科,乌鲁木齐 841100 ;2.省部共建中亚高发病成因与防治国家重点实验室, 乌鲁木齐 841100)
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| 摘要: |
| 基于公共数据库探究食管鳞癌(Esophageal squamous cell carcinoma,ESCC)中差异表达的类泛素化调节因子及潜在的功能。从TCGA数据库中获取1个食管鳞癌数据集并进行类泛素化调节因子的差异分析。用单因素Cox回归和套索回归(LASSO)筛选与预后相关的关键基因。利用Cox回归构建预后模型评估关键基因的独立预后价值。通过收集食管鳞癌患者癌组织与癌旁组织行RT-qPCR分析,初步验证筛出的差异基因。利用Gene MANIA 数据库对关键基因绘制蛋白质互作图(PPI),对网络中的相关基因进行基因功能和通路富集分析。基于TIMER算法进行关键基因和免疫细胞浸润的相关性研究。结果得到2个与食管鳞状细胞癌预后相关的类泛素化调节因子(DESI2和SMC6)。RT-qPCR结果提示 DESI2和SMC6在ESCC中高表达(P＜0.05)。PPI筛出的核心基因功能和富集分析表明,其可能涉及非同源末端结合和同源性重组加速DNA修复。最后通过TIMER算法发现DESI2和SMC6与肿瘤免疫浸润相关。结果提示DESI2和SMC6在ESCC中的表达具有明显的异质性,可能参与肿瘤进展,为ESCC发病机制、临床诊断和治疗靶点的研究提供理论依据。 |
| 关键词: 食管鳞状细胞癌 类泛素化 免疫细胞浸润 生物信息学 |
| DOI：10.12113/202405011 |
| 分类号:R73 |
| 文献标识码:A |
| 基金项目:新疆维吾尔自治区省部共建中亚高发病成因与防治项目(SKL-HIDCA-2020-SG5). |
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| Screening and bioinformatics analysis of differential ubiquitinizationregulators in esophageal squamous cell carcinoma |
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SUN Qingchao1,2, JIN Weiming1, ZHANG Liwei1, LI Shaojie1, LI Desheng1,2
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(1.Department of Thoracic Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 841100, China; 2.State Key Laboratory of Central Asia High, Urumqi 841100, China)
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| Abstract: |
| To investigate differentially expressed class ubiquitin-like modifier (Ub-like) regulatory factors and their potential functions in esophageal squamous cell carcinoma (ESCC) based on public databases, we conducted a study. We obtained one ESCC dataset from the TCGA database for Ub-like regulatory factor differential analysis. We used univariate Cox regression and LASSO regression to screen key genes related to prognosis. We used Cox regression to construct a prognostic model to evaluate the independent prognostic value of the key genes. We used RT-qPCR to initially validate the differential genes in ESCC patients by collecting cancer tissues and adjacent tissues. We used Gene MANIA database to draw a protein-protein interaction (PPI) map for the key genes and performed gene function and pathway enrichment analysis on the networks related genes. We studied the correlation between key genes and immune cell infiltration using the TIMER algorithm. Our study identified two Ub-like regulatory factors, DESI2 and SMC6, that were related to the prognosis of ESCC. The RT-qPCR results showed that DESI2 and SMC6 were highly expressed in ESCC(P<0.05). The core genes identified by PPI and their functional and enrichment analysis suggested that they may be involved in non-homologous end joining and homologous recombination-accelerated DNA repair. Finally, we found that DESI2 and SMC6 were related to immune cell infiltration using the TIMER algorithm. The results suggest that the expression of DESI2 and SMC6 in ESCC has significant heterogeneity and may be involved in tumor progression, providing theoretical basis for the study of the pathogenesis, clinical diagnosis, and therapeutic targets of ESCC. |
| Key words: Esophageal squamous cell carcinoma SUMOylation Immune cell infiltration Biological information science |
