| 引用本文: | 刘益文,张晓雯,高邦婷,项钦戈,张晨阳,左成,杨欢,汪大巍,张高博.柯萨奇病毒A组6型VP1的主要特性[]及表位预测分析[J].生物信息学,2025,23(1):50-60. |
| LIU Yiwen,ZHANG Xiaowen,GAO Bangting,XIANG Qinge,ZHANG Chenyang,ZUO Cheng,YANG Huan,WANG Dawei,ZHANG Gaobo.Predictive analysis of main characteristics and epitopes of VP1 from Coxsackievirus A6[J].Chinese Journal of Bioinformatics,2025,23(1):50-60. |
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| 柯萨奇病毒A组6型VP1的主要特性[]及表位预测分析 |
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刘益文1, 张晓雯1 ,高邦婷1 ,项钦戈1 ,张晨阳1 ,左成1 ,杨欢1,汪大巍1 ,张高博2
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(1.武汉华夏理工学院,武汉 430223;2.湖北葛店人福药用辅料有限责任公司,湖北 鄂州 436070)[HJ1.5mm]
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| 摘要: |
| 运用生物信息学方法预测分析襄阳地区分离的柯萨奇病毒A组6型(Coxsackievirus A6, CV-A6)病毒蛋白(Viral protein, VP)中VP1的主要特性及表位。应用ProtParam,SOPMA,Phyre 2,DNAstar v8.1.3,Mega11,ABCpred,ElliPro,NetMHCpan-4.1,NetMHCIIpan-4.0等软件和在线网站预测分析CV-A6 VP1包括理化性质、结构特征、序列特点,亲缘关系在内的主要特性及B,T细胞抗原表位。结果发现该分离株VP1为碱性、不稳定的亲水性蛋白,其二级结构以无规则卷曲为主,核苷酸(氨基酸)同源性不一,有12个氨基酸突变位点,属于D3a亚型。该蛋白还存在多个潜在及优势B,T细胞抗原表位。CV-A6襄阳分离株与国内广西、广东地区CV-A6共进化共循环,具有一定的免疫潜能,可为分子流行病学监测以及多价疫苗的研制提供科学依据与参考。 |
| 关键词: CV-A6 VP1 生物信息学 主要特性 表位 |
| DOI：10.12113/202306004 |
| 分类号:R373.2+3 |
| 文献标识码:A |
| 基金项目:武汉华夏理工学院校级科研基金项目(No.22011). |
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| Predictive analysis of main characteristics and epitopes of VP1 from Coxsackievirus A6 |
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LIU Yiwen1, ZHANG Xiaowen1, GAO Bangting1, XIANG Qinge1, ZHANG Chenyang1, ZUO Cheng1, YANG Huan1, WANG Dawei1, ZHANG Gaobo2
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(1. Wuhan Huaxia Institute of Technology, Wuhan 430223,China; 2. Hubei Gedian Humanwell Pharmaceutical Excipients Co.,Ltd., Ezhou 436070, Hubei,China)
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| Abstract: |
| To predict and analyze the main characteristics and epitopes of VP1 in the viral protein (VP) of Coxsackievirus A6 (CV-A6) isolated from Xiangyang area using bioinformatics methods. The CV-A6 VP1s main characteristics including physicochemical properties, structural features, sequence characteristics, affinity and B/T cell epitopes are predicted and analyzed by software and online sites such as ProtParam, SOPMA, Phyre 2, DNAstar v8.1.3, Mega11, ABCpred, ElliPro, NetMHCpan-4.1, NetMHCIIpan-4.0. The isolate, VP1, is a basic, unstable, hydrophilic protein which has the secondary structure mainly consists of random coil, disparate nucleotide (amino acid) homology, 12 amino acid mutation sites, and belongs to the D3a isoform. There are also multiple potential and dominant B/T cell epitopes for VP1. The CV-A6 Xiangyang isolate co-evolved and co-circulated with CV-A6 in Guangxi and Guangdong, China, and has certain immune potential, which can provide scientific basis and reference for molecular epidemiological surveillance and the development of multivalent vaccines. |
| Key words: CV-A6 VP1 Bioinformatics Main characteristic Epitope |
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