| 摘要: |
| 泛素羧基末端水解酶-1(ubiquitin C-terminal hydrolase L1, UCHL1)是一种去泛素化酶,特异性表达于脑与生殖腺,具有去泛素化活性、稳定细胞内泛素单体的功能。为确定UCHL1在脊椎动物中是否普遍存在,从而选择身体构造更简单的模式动物研究其生物学功能,通过生物信息学手段分析脊椎动物门的11种生物中基因UCHL1的分子进化情况,分析显示UCHL1基因组长度在进化过程中变化较大,但外显子个数变化较小,蛋白氨基酸残基数目也基本维持在223 aa左右。分析结果表明,mRNA有不同的剪接方式,但氨基酸序列在进化上是高度保守的,UCHL1蛋白活性位点同源性高达90%,该结果证明了蛋白UCHL1的功能对于物种正常生存是必须的。 |
| 关键词: UCHL1 去泛素化 进化 保守性 同源性 |
| DOI：10.3969/j.issn.1672-5565.201701002 |
| 分类号:Q349+.55 |
| 文献标识码:A |
| 基金项目:国家自然科学基金(2,9). |
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| Molecular evolution analysis of ubiquitin C-terminal hydrolase L1 (UCHL1) |
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ZHANG Yu1,2, SUN Zhaogui2
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(1.Shanghai Medical College, Fudan University, Shanghai 200032,China; 2.National Population and Family Planning Key Laboratory of Contraceptive Drugs & Devices(Shanghai Institute of Planned Parenthood Research), Shanghai 200032,China)
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| Abstract: |
| UCHL1 (ubiquitin C-terminal hydrolase L1) is one of the deubiquitinases, which is specifically expressed in the brain and gonad. It has the function of deubiquitination, and can maintain a stable pool of monoubiquitin in cells.To determine whether UCHL1 is ubiquitous in vertebrates, and further select model animals with simpler body structure to study biological functions of UCHL1 accordingly. The molecular evolution of UCHL1 gene in 11 species of vertebrates is analyzed by bioinformatics methods. The analysis results show that the genomic length of UCHL1 gene is changed greatly in evolution, but the number of exons is changed little, and the number of amino acids is maintained at about 223. The above results indicate that mRNA has different splicing patterns, but the amino acid sequence is highly conserved in evolution, and the homology of UCHL1 active sites is as high as 90%. These results indicate that the function of UCHL1 is necessary for the survival of the species. |
| Key words: UCHL1 Deubiquitination Evolution Conservation Homology |
