| 引用本文: | 高波,王伟,钱跃,陈才,钟继汉,沈丹,陈伟,宋成义.斑马鱼转座子时空表达特性[J].生物信息学,2017,15(4):201-206. |
| GAO Bo,WANG Wei,QIAN Yue,CHEN Cai,ZHONG Jihan,SHEN Dan,CHEN Wei,SONG Chengyi.Temporal and spatial expression characteristics of transposons in Zebrafish[J].Chinese Journal of Bioinformatics,2017,15(4):201-206. |
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| 斑马鱼转座子时空表达特性 |
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高波,王伟,钱跃,陈才,钟继汉,沈丹,陈伟,宋成义
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(扬州大学 动物科学与技术学院,江苏 扬州 225009)
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| 摘要: |
| 转座子是基因组中可移动和扩展的元件,能够插入新的位点,影响基因组和基因的结构和功能,是基因组进化的内在驱动。为探讨转座子的时空表达特性,首先通过生物信息学方法鉴定出斑马鱼9个疑似活性转座子,包括DNA转座子Tc1家族(Tc-a、Tc-b、Tc-c、Tc-d、Tc-e)、反转录转座子ERV家族(ERV-1、ERV-2)和LINE家族(L1-323、L1-21),然后采用实时荧光定量PCR法检测上述转座子在斑马鱼早期胚胎发育7个阶段及成鱼各主要脏器的表达活性。结果表明:Tc1家族在0.75、2.00、3.00 h各阶段无转录活性,在6.00、15.00、24.00、48.00 h各阶段各转座子均有较高转录活性；反转录转座子转录活性最早出现于3 h,最晚出现于15 h,且随着发育时间的延长,转录活性显著增强。9种转座子在成鱼心脏、大脑、肌肉、肝脏、睾丸和卵巢均有表达,且大脑和心脏中的表达水平显著高于其他组织,睾丸表达水平最低。分析表明转座子的表达具有时间和组织的特异性,可能参与斑马鱼胚胎和组织器官发育调控,尤其是大脑和心脏发育。这些结果为进一步研究转座子是否具有基因表达调控功能提供重要参考。 |
| 关键词: 转座子 Tc1家族 ERV家族 LINE家族 时空表达 斑马鱼 |
| DOI：10.3969/j.issn.1672-5565.201703004 |
| 分类号:Q344 |
| 文献标识码:A |
| 基金项目:国家自然科学基金(3,4);扬州市现代农业项目(YZ2016040). |
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| Temporal and spatial expression characteristics of transposons in Zebrafish |
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GAO Bo, WANG Wei, QIAN Yue, CHEN Cai, ZHONG Jihan, SHEN Dan, CHEN Wei, SONG Chengyi
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(College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangshu, China)
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| Abstract: |
| Transposons are DNA sequences capable of moving and expansion in genomes. They can insert new sites, influence the structure and function of genes and genomes, and play important roles in genome evolution. We identified 9 suspected active transposons in zebrafish, including Tc1 family (Tc-a, Tc-b, Tc-c, Tc-d, Tc-e) from DNA transposons, ERV and Line families (ERV-1, ERV-2, L1-323, L1-21) retrotransposon. To explore the expression pattern of transposons, the transcription activity of 9 transposons in 7 stages of zebrafish embryo and adult viscera were assayed by real time quantitative PCR. The results showed Tc1 transposons didnt transcript at stages of 0.75 h, 2.00 h and 3.00 h, but had high transcription activity at 6.00 h, 15.00 h, 24.00 h and 48.00 h; retrotransposons started to transcript at 3.00 h or 15.00 h stage. The expression level became stronger with development. 9 transposons were all expressed in heart, brain, muscle, live, testis and ovary. The expression level in brain and heart were significant higher than other tissues, and that in testis was lowest. These data suggest that the transposons have specifically-temporal and spatial expression profile. Transposable elements may play a role during embryonic and tissue development, especially in brain and heart development, which also provide a solid experimental foundation for the research of gene expression and regulation of transposons. |
| Key words: Transposon Tc1 family ERV family Line family Temporal and spatial expression profile Zebrafish |
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