| 摘要: |
| 自噬对肝再生有积极的作用,但具体作用机制仍有待阐明。为了解自噬在大鼠肝再生的变化和机理,通过蛋白质组学(iTRAQ方法)检测了大鼠肝再生中调控自噬的信号通路相关蛋白和自噬过程相关蛋白的变化。结果表明,调控自噬的PI3K/Akt,mTOR,AMPK均被激活,泛素-蛋白酶体相关蛋白发生显著表达变化,溶酶体相关膜蛋白和水解酶发生显著变化。IPA分析发现,自噬在肝再生的启动阶段和进展阶段上调。根据研究结果,提出线粒体和溶酶体共存假说,并初步探讨并图示其存在的可能性和机理。 |
| 关键词: 肝再生 自噬 泛素蛋白酶体 线粒体自噬 线粒体溶酶体共存 |
| DOI：10.3969/j.issn.1672-5565.20161222001 |
| 分类号:Q2 |
| 文献标识码:A |
| 基金项目:国家自然科学基金(31201093);国家重点基础研究发展计划(973计划)(2012CB722304). |
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| Preliminary research on the role of autophagy in rat liver regeneration |
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YIN Li1,2,3, XU Cunshuan1,2
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(1.College of Life Science, Henan Normal University, Xinxiang 453007, Henan, China; 2.State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Bioengineering Key Laboratory(Henan Normal University), Xinxiang 453007, Henan,China; 3. Luohe Medical College, Luohe 462002, Henan, China)
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| Abstract: |
| Autophagy affects the liver regeneration (LR). However, the detailed mechanism remains to be elucidated. In order to explore the change and mechanism of autophagy in rat LR, we detected the expression changes of proteins in the signal pathways which regulate autophagy and the proteins associated with autophagy process by isobaric tags for relative and absolute quantitation (iTRAQ) combined with mass spectrometry (MS). The results indicated that the PI3K/Akt, mTOR and AMPK signaling pathways were activated. The ubiquitin-proteasome-related proteins and the membrane proteins and hydrolases associated with lysosome changed significantly. Autophagy up-regulated at the priming and progression stage in LR by the analysis from IPA. We proposed the hypothesis that lysosomes and mitochondria might coexist and explore the possible mechanism about that kind of coexistence. |
| Key words: liver regeneration autophagy ubiquitin-proteasomes system (UPS) mitophagy coexistence of mitochondria and lysosome ATP release |
