| 引用本文: | 李惠敏,罗琼,肖君,李少梅,刘华英,秦新民.沙田柚RING-H2 finger基因的生物信息学分析[J].生物信息学,2017,15(3):149-155. |
| LI Huimin,LUO Qiong,XIAO Jun,LI Shaomei,LIU Huaying,QIN Xinmin.Bioinformatics analysis of RING-H2 finger gene from Citrus grandisvar. ShatianyuHort[J].Chinese Journal of Bioinformatics,2017,15(3):149-155. |
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| 摘要: |
| 为探讨沙田柚自交不亲和性的机理,利用高通量测序技术对沙田柚自交和异交花柱进行转录组测序,通过差异分析得到了沙田柚RING-H2 finger基因序列。采用生物信息学方法,对其编码的蛋白质从序列特征、理化性质、跨膜结构域、高级结构以及功能域等方面进行了预测和分析。结果表明:该基因全长为845 bp,开放阅读框(ORF)全长为387 bp,编码128个氨基酸,编码蛋白质的分子量为13.61 KDa,理论等电点为4.26； 该蛋白质含有一个植物RING-H2 finger蛋白家族的保守结构域,为疏水性不稳定蛋白。氨基酸序列分析表明,其编码的氨基酸与甜橙(Cs4g15340.1)RING-H2 finger 蛋白的相似度为98%。这些分析结果可为今后深入研究该蛋白的结构特征和功能提供参考。 |
| 关键词: 沙田柚 RING-H2 finger蛋白基因 生物信息学 环指结构域 |
| DOI：10.3969/j.issn.1672-5565.201701005 |
| 分类号:S576 |
| 文献标识码:A |
| 基金项目:国家自然科学基金(31360477)；广西高校科研项目(2013YB036). |
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| Bioinformatics analysis of RING-H2 finger gene from Citrus grandisvar. ShatianyuHort |
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LI Huimin, LUO Qiong, XIAO Jun, LI Shaomei, LIU Huaying, QIN Xinmin
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(College of Life Science, Guangxi Normal University, Guilin 541004,Guangxi,China)
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| Abstract: |
| To explore the molecular mechanism of self-incompatibility,the transcriptomes of the self-pollinated style and cross-pollinated style of Citrus grandisvar. ShatinyuHort were sequenced by high-throughput sequencing technology. RING-H2 finger gene sequence of Citrus grandisvar. ShatinyuHort was obtained through differential analysis method, and some characters of the RING-H2 finger protein were analyzed and predicted by bioinformatics method, including the composition of amino acid sequence, physicochemical parameters, hydrophobicity, transmembrane domain, structure and function of protein etc. The results showed that RING-H2 finger gene was 845 bp in length with an open reading frame(ORF) of 387 bp, encoding 128 amino acids with deduced molecular weight of 13.61 KDa, and theoretical pI value of 4.26, and contained a RING-H2 finger domain. Bioinformatics analysis showed that RING-H2 finger protein was a hydrophobic and unstable protein. The homology analysis of amino acid sequence indicated that the RING-H2 finger protein shared high similarity with RING-H2 finger protein of Citrus sinensis Cs4g15340.1 (98%).This work will provide the useful reference for further investigation of the structure and function of the RING-H2 finger protein． |
| Key words: Citrus grandisvar. ShatinyuHort RING-H2 finger protein gene bioinformatics RING finger domain |
