| 引用本文: | 单增辉,丰继华,陈攀峰,魏恨恨,胡焕.基于DNA弯曲度的H2A.Z核小体定位与修饰研究[J].生物信息学,2015,13(1):13-17. |
| SHAN Zenghui,FENG Jihua,CHEN Panfeng,WEI Henhen,HU Huan.Based on the DNA bending H2A. Z nucleosome positioning and modification research[J].Chinese Journal of Bioinformatics,2015,13(1):13-17. |
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| 摘要: |
| 在真核生物染色质中,H2A.Z是高度保守的组蛋白变异体, 与转录调控、基因组的稳定性密切相关。为了探讨组蛋白修饰、DNA弯曲度与H2A.Z核小体定位三者之间的关联,在得到实验所测的相关数据后,利用MINE算法并结合皮尔逊相关系数在酵母全基因组的转录起始位点周围探讨了三者间的线性与非线性关系。其中MIC算法可以定量的得出数据之间关联度大小的值,用于衡量数据之间是否存在着关联,而皮尔逊相关系数则用于检查是否为线性关联。结果除了发现大部分组蛋白修饰种类和核小体定位之间存在着线性关联外,还探测到有两种组蛋白修饰数据(H4ac修饰与GCN4修饰)和核小体定位数据之间存在着以往未发现的非线性关系(大致呈正余弦函数),并从数据的生物背景(组蛋白修饰与核小体位置)上探讨了出现非线性现象的原因。 |
| 关键词: 转录起始位点(TSS) 组蛋白变异体 H2A.Z 修饰 核小体 |
| DOI：10.3969/j.issn.1672-5565.2015.01.03 |
| 分类号:Q-3 |
| 基金项目:国家自然科学基金项目(31160234)；云南省应用基础研究计划项目(2011FB082)。 |
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| Based on the DNA bending H2A. Z nucleosome positioning and modification research |
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SHAN Zenghui,FENG Jihua, CHEN Panfeng, WEI Henhen, HU Huan
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(School of Electrical and Information Technology, Yunnan University of Nationalities, Kunming 650500,China)
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| Abstract: |
| In eukaryotes chromatin, H2A.Z is highly conservative histone variants and closely associated with the transcriptional regulation and the stability of the genome and of high importance. In order to explore the links among the histone modification, DNA bending and H2A.Z nucleosome positioning. After getting the relevant data sets, we discussed the linear and nonlinear relationships between those datas around transcription start site in the yeast genome-wide by MIC algorithm and Pearson correlation coefficient. The MIC algorithm got a correlation value to quantificationally measure whether there is an association between datas, while pearson correlation coefficient is used to check whether the correlation is linear. The results showed most of the types of histone modification were linear correlation between the nucleosome positioning. In addition, two kinds of histone modification datas (H4ac modification with GCN4 modification) were found between nucleosome positioning(roughly is positively cosine function) and discussed the reasons of nonlinear phenomena from the biological background(histone modification and nucleosome position) of datas. |
| Key words: TSS Histone variants H2A.Z Modify Nucleosome |
