| 引用本文: | 刘巧红,罗成飞,代翠红,袁肖寒,梁乃国,刘天骄,史淑芝,于歆,吕星道.甜菜属厚皮菜SWTY-1细胞质VNTRs多态性分析[J].生物信息学,2015,13(1):9-12. |
| LIU Qiaohong,LUO Chengfei,DAI Cuihong,YUAN Xiaohan,LIANG Naiguo,LIU Tianjiao,SHI Shuzhi,YU Xin,LV Xingdao.Analysis for variable number of tandem repeats in cytoplasm genome of Beta vulgaris ciclaSWTY-1[J].Chinese Journal of Bioinformatics,2015,13(1):9-12. |
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| 甜菜属厚皮菜SWTY-1细胞质VNTRs多态性分析 |
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刘巧红1,罗成飞1,代翠红1,袁肖寒2,梁乃国1,刘天骄1,史淑芝1,于歆1,吕星道2
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( 1.哈尔滨工业大学食品科学与工程学院,哈尔滨150090; 2.东北农业大学生命科学与生物技术研究中心,哈尔滨150030 )
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| 摘要: |
| 利用数目可变串联重复序列(Variable Number of Tandem Repeats,VNTRs) 微卫星标记方法,对重庆厚皮菜甜菜材料SWTY-1群体中100个单株的细胞质线粒体DNA片段中TR2位点VNTRs片段多态性进行分析。结果显示97个单株线粒体TR2位点微卫星串联重复序列均为3拷贝,与普通糖甜菜一致；3个单株线粒体TR2位点微卫星串联重复序列为6拷贝,发现甜菜属厚皮菜细胞质TR2位点VNTRs存在多态性,在该群体中发现了不同于甜菜栽培种新的细胞质单株。对该群体材料100个单株的抽薹及结籽进行观测,结果显示微卫星串联重复序列为6拷贝的变异植株中2个单株花期未抽苔开花,1株抽苔晚未形成正常种子；细胞质TR2位点VNTRs片段拷贝数为3的植株中2个单株未能正常抽薹,其他植株均正常抽薹结籽。 |
| 关键词: 甜菜属 细胞质 雄不育 线粒体 分子标记 |
| DOI：10.3969/j.issn.1672-5565.2015.01.02 |
| 分类号:TS242.2 |
| 基金项目:国家自然科学基金(No.31371685) 。 |
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| Analysis for variable number of tandem repeats in cytoplasm genome of Beta vulgaris ciclaSWTY-1 |
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LIU Qiaohong1, LUO Chengfei1, DAI Cuihong1,YUAN Xiaohan2,LIANG Naiguo1, LIU Tianjiao1, SHI Shuzhi1,YU Xin1,LV Xingdao2
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(1.School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China; 2.Life Science and Biotechnique Research Center, Northeast Agricultural University, Harbin 150090, China)
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| Abstract: |
| Molecular identification for cytoplasm polymorphism of Beta vulgaris cicla Lmaterials SWTY-1 was conducted in this paper. Polymorphisms in mtDNA TR2 locus of Beta vulgaris ciclaSWTY-1 were analyzed using variable number of tandem repeats (VNTRs) molecular markers method. The result showed that the numbers of tandem repeats in mtDNA TR2 locus were three-copy array of tandem repeats in 97 single plants among the 100 plants of Swiss chard, which was consistent with sugar beet. Six-copy VNTRs in mtDNA TR2 locus were found in 3 single plants among the population. Two plants could not bolt among the three plants whose VNTRs copy numbers were six. One plant bolting late could not form normal seeds among the three plants whose VNTRs copy number were six. Only two plant among the 97 plants with three-copy in TR2 locus failed to bolt normally and others bolt normally and form seeds. |
| Key words: Beta Cytoplasm Male sterility mt DNA Molecular markers |
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