| 甘蔗MYB2转录因子的电子克隆和生物信息学分析 |
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| 引用本文： | 李国印,阙友雄,许莉萍,郭晋隆,闫学兵,陈如凯.甘蔗MYB2转录因子的电子克隆和生物信息学分析[J].生物信息学,2011,9(1):24-27. |
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| 作者姓名： | 李国印 阙友雄 许莉萍 郭晋隆 闫学兵 陈如凯 |
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| 作者单位： | 福建农林大学,农业部甘蔗遗传改良重点开放实验室,福建,福州,350002 |
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| 基金项目： | 国家948项目(2010-C21) |
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| 摘 要： | 用电子克隆方法获得甘蔗MYB2基因,采用生物信息学方法,对该基因编码蛋白从氨基酸组成、理化性质、跨膜结构域、疏水性/亲水性、亚细胞定位、高级结构及功能域等方面进行了预测和分析。结果表明:甘蔗MYB2基因全长991bp,包含570bp的ORF,编码189个氨基酸。甘蔗MYB2基因包含有MYB功能域,在序列组成、高级结构及活性位点等方面,与玉米等其它植物的MYB2基因具有高度的相似性。研究结果为该基因的实验克隆奠定基础。
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| 关 键 词： | 甘蔗 MYB2基因 电子克隆 生物信息学 |
Electronic cloning and characterization of MYB2 gene from Saccharum officinarum using bioinformatics tools |
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| LI Guo-yin,QUE You-xiong,XU Li-ping,GUO Jin-long,YAN Xue-bing,CHEN Ru-kai.Electronic cloning and characterization of MYB2 gene from Saccharum officinarum using bioinformatics tools[J].China Journal of Bioinformation,2011,9(1):24-27. |
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| Authors: | LI Guo-yin QUE You-xiong XU Li-ping GUO Jin-long YAN Xue-bing CHEN Ru-kai |
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| Affiliation: | LI Guo-yin,QUE You-xiong,XU Li-ping,GUO Jin-long,YAN Xue-bing,CHEN Ru-kai (Key Laboratory of Sugarcane Genetic Improvement,Ministry of Agriculture,Fujian Agriculture&Forestry University,Fuzhou 350002,China) |
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| Abstract: | An novel MYB2 gene from Saccharum officinarum was cloned in silico based on the EST seqences from Unigene of NCBI.Some characters of the MYB2 encodes amino acid were analyzed and predicted by the tools of bioinformatics in the following aspects,including the compositon of amino acid sequence,hydrophobicity or hydrophilicity,secondary and tertiary structure of protein and funcion.Bioinformatical analysis showed that the full-length of MYB2 gene from S.officinarum was 991 bp and it contained a complete ORF wh... |
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| Keywords: | Saccharum officinarum MYB2 gene In silico cloning Bioinformatics |
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