| 一株氯氰菊酯降解菌16SrDNA，gyrB和GyrB的系统发育分析 |
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| 引用本文： | 张久刚,闫艳春.一株氯氰菊酯降解菌16SrDNA，gyrB和GyrB的系统发育分析[J].生物信息学,2008,6(2):55-58. |
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| 作者姓名： | 张久刚 闫艳春 |
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| 作者单位： | 1. 中国农业科学院,研究生院,北京,100081
2. 中国农业科学院,研究生院,北京,100081;中国农业科学院,研究生院,北京,100081 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 从农药厂污水处理池中分离得到一株氯氰菊酯降解菌，在30℃，pH7．0的条件下，无机盐培养基中100mg／L的氯氰菊酯，经过7．5天，能够降解大约52．3％，外加碳源能够明显提高其降解性能。生理生化实验结合16SrDNA，册诏和GyrB的系统发育分析，将其归为Gordonia菌属。在16SrDNA水平上，其与G．amicalis DSM44461和G．hydrophobica DSM44015^T的相似值最高，为98．1％；而在gyrB和GyrB水平上，其与G.hydrophobica JCM10086的相似值最高，分别为86．8％和91，1％。通过对所构建的系统发育树进行评估，表明16SrDNA序列适用于将分离菌株鉴定到属的水平上，而gyrB和GyrB更适用于在属内种的水平上进行系统发育的分析。
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| 关 键 词： | 氯氰菊酯 生物降解 系统发育分析 |
| 文章编号： | 1672-5565(2008)-02-55-04 |
| 修稿时间： | 2007年3月9日 |
Phylogenetic assessment of a new cypermethrin- degrading bacterium Gordonia sp.D2 based on 16S rDNA, gyrB and GyrB sequences |
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| ZHANG Jiu-gang,YAN Yan-chun.Phylogenetic assessment of a new cypermethrin- degrading bacterium Gordonia sp.D2 based on 16S rDNA, gyrB and GyrB sequences[J].China Journal of Bioinformation,2008,6(2):55-58. |
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| Authors: | ZHANG Jiu-gang YAN Yan-chun |
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| Affiliation: | ZHANG Jiu - gang, YAN Yan - chun ( 1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai＇ an 271018, china; 2. Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, china ) |
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| Abstract: | A cypermethrin - degrading bacterium named as Gordonia sp. D2 was isolated from activated sludge of a wastewater treatment biore-actor. At 30 ℃ and pH 7.0, the removal rate of 100 mg cypennethrin L-1 was approximately 52.3% in mineral salts medium within 7.5 days, and extra carbon source addition could enhance the biodegradation. The isolate was identified as Gordonia genus through physio - bio- chemical identification combined with the phylogenetic analyses based on 16S rRNA, gyrB nucleotide and GyrB amino acid sequences. 16S rRNA sequences of strain D2 showed the highest similarity to G. amicalis DSM44461 and G. hydrophobica DSM44015T, with the same value of 98.1% ,meanwhile, D2 shows the highest similarity to G. hydrophobica JCA10086 with the value of 86.8% and 91.1% based on gyrB and GyrB. Three phylogenetic dendrograms were made, and results showed that 16S rRNA gene is suit for identifying the isolate to the genus level, gyrB gene and GyrB amino acid sequences are more adapted for phylogenetic analysis. |
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| Keywords: | cypermethrin biodegradation phylogenetic analysis |
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