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| 茂原链霉菌谷氨酰胺转胺酶序列分析及三维结构建模 | |
| 引用本文： | 张智俊,田兴军,李亚玲,罗淑萍.茂原链霉菌谷氨酰胺转胺酶序列分析及三维结构建模[J].生物信息学,2005,3(3):97-100,120. |
| 作者姓名： | 张智俊 田兴军 李亚玲 罗淑萍 |
| 作者单位： | 1. 南京大学生命科学院,南京,210093 2. 东胜创新生物科技有限公司,南京,210090 3. 新疆农业大学农学院,乌鲁木齐,830052 |
| 基金项目： | 中国博士后科学基金(0208003034)，教育部博士点基金(20030284044) |
| 摘 要： | 利用DNASTAR、VectorNTI9、SignalP等生物信息学平台对茂原链霉菌(Streptomyces mobaraensis)谷氨酰胺转胺酶(TGase)进行了序列分析和三维结构建模。结果表明:茂原链霉菌TGase同已报道其他微生物来源的TGase有较高的同源性,无信号肽,存在一个跨膜结构区,二级结构是由多个α螺旋、转角和少量β折叠构成。同时在一二级结构分析的基础上,利用同源建模的方法完成了三维结构的建模。 |
| 关 键 词： | 序列分析 谷氨酰胺转胺酶 三维结构同源建模 茂原链霉菌 |
| 文章编号： | 1672-5565（2005）-03-97-05 |
| 收稿时间： | 2004-09-22 |
| 修稿时间： | 2005-05-19 |
Sequence analysis and three- dimensional structure modeling of transglutaminase from streptomyces mobaraensis |
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| ZHANG Zhi-Jun,TIAN Xing-Jun,LI Ya-ling,LUO Shu-Ping.Sequence analysis and three- dimensional structure modeling of transglutaminase from streptomyces mobaraensis[J].China Journal of Bioinformation,2005,3(3):97-100,120. | |
| Authors: | ZHANG Zhi-Jun TIAN Xing-Jun LI Ya-ling LUO Shu-Ping |
| Abstract: | Sequence analysis of Transglutaminase from streptomyces mobaraensis was conducted by bioinformatic tools such as DNAstar,Vector NTI and SignalP etc.. The results show that the TGase obtained from S.mobaraensis in this study had higher similarity with reported microbial TGase,contained a transmemberane regions but no signal peptide and its secondary structure was composed of many alpha regions,turns regions and few beta regions. Based on the primary and secondary structure analysis of TGase, Three-dimensional structure of TGase was developed by homology modeling methods. |
| Keywords: | Sequence Analysis Transglutaminase Homology Modeling Streptomyces mobaraensis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
