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稻瘟菌Ⅰ型烯醇化酶基因全长cDNA的电子克隆
引用本文:张会敏,姜明国,冯友军.稻瘟菌Ⅰ型烯醇化酶基因全长cDNA的电子克隆[J].生物信息学,2006,4(2):57-61.
作者姓名:张会敏  姜明国  冯友军
作者单位:1. 香港基因有限公司,北京,100053
2. 广西民族大学,化学与生态工程学院,广西,南宁,530004
3. 中国科学院微生物研究所,北京,100080
摘    要:利用电子克隆技术从稻瘟菌中克隆到一个新的Ⅰ型烯醇化酶全长cDNA,暂命为MgEno-1。MgEno-1全长1571核薯酸,其预测的ORF为1317核苷酸,共编码438个氨基酸。起始密码子ATG位于第53位,终止密码子TAA位于第1369位。序列分析表明该烯醇化酶与丝状真菌中已报道的其它烯醇化酶高度同源,且长度一致,这暗示烯醇化酶基因进化上高度保守,甚至有可能像18SrRNA一样可作为进化尺度。这将是第一个用电子克隆技术从稻瘟菌中克隆到的基因。

关 键 词:电子克隆  烯醇化酶  稻瘟菌
文章编号:1672-5565(2006)-02-0057-05
收稿时间:2004-09-30
修稿时间:2005-11-04

In silico cloning of MgEno - 1 cDNA from Magnaporthe grisea
Zhang Hui-min,Jiang Ming-guo,Feng You-jun.In silico cloning of MgEno - 1 cDNA from Magnaporthe grisea[J].China Journal of Bioinformation,2006,4(2):57-61.
Authors:Zhang Hui-min  Jiang Ming-guo  Feng You-jun
Abstract:Here we report a novel cDNA clone( MgEno - 1 )encoding α - enolase in rice blast fungus, Magnaporthe grisea ( M. grisea ), acquired by in silico cloning strategy. The MgEno- 1 consists of 1571 base pairs(bp)in length, and was predicted to contain a 1317 bp of open reading framework(ORF) corresponding to 438 deduced amino acids(aa) ,with ATG initial codon and TAA stopping codon at the position of 53and 1369 nucleotide acid, respectively. Sequence analysis of the predicted ORF of MgEno - 1 revealed that it is of the nearly equal length and of high identity to enolase- 1 genes published in the filamentous fungi, implying that it is highly conservative, and even has the potential to serve an evolutionary scale like 18S rRNA. To our knowledge, it will be the first gene cloned from M. grisea using the technique of in silico cloning.
Keywords:In silico cloning  Enolase  Magnaporthe grisea
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