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基于电子克隆方法获得鸡MIBP全长cDNA
引用本文:高兴春,顾雪峰,韩俊,朱大海.基于电子克隆方法获得鸡MIBP全长cDNA[J].生物信息学,2005,3(2):58-61,88.
作者姓名:高兴春  顾雪峰  韩俊  朱大海
作者单位:1. 哈尔滨工业大学生命科学系,哈尔滨,150001
2. 哈尔滨工业大学生命科学系,哈尔滨,150001;哈尔滨基太生物芯片开发有限责任公司,哈尔滨,150090
3. 哈尔滨基太生物芯片开发有限责任公司,哈尔滨,150090
摘    要:随着生物数据的急剧增长和计算机技术的快速提高,生物信息学这一新兴学科得到了前所未有的迅速发展,应用的领域越来越广。应用电子克隆技术来寻找未知新基因,就是生物信息学在全长新基因发现上的具体应用。电子克隆与其他发现全长基因的方法相比可以充分利用已有的数据库资源,避免大量的重复性劳动,节省时间和开支,加快新基因的发现。在鸡的大规模cDNA克隆测序的背景下,利用EST数据库比对,应用电子克隆技术来寻找鸡的末知新基因。并成功的预测了一个EST的全长cDNA序列物(947bp),同时对其进行了蛋白质水平的预测与分析,被步确定它编码206个氨基酸,其蛋白质序列与人肉整合素结合蛋白β1具有较高相似性(相似性为73%)。经RT-PCR扩增获得预期片断,测序鉴定,命名为ChickenMIBP。

关 键 词:电子克隆    MIBP
文章编号:1672-5565(2005)-02-0058-05
修稿时间:2004年10月27

Clone full - length cDNA for chicken MIBP by silico cloning
Gao Xin-chun,GU Xue-feng ,Han Jun.Clone full - length cDNA for chicken MIBP by silico cloning[J].China Journal of Bioinformation,2005,3(2):58-61,88.
Authors:Gao Xin-chun  GU Xue-feng    Han Jun
Affiliation:Gao Xin-chun1,GU Xue-feng 1,2,Han Jun2
Abstract:As the biological information is exploding and the computer technology is improving rapidly,Bioinformatics as a novel subject develops at such a speed that is highest in its history.It is applied in more and more fields.Using in silico cloning to identify unknown gene is a concrete application of Bioinformatics in the full-length novel genes discovery.Compared with other ways,this technology makes best use of existing database to avoid much of repetitive work,and saves time and expenditure,which makes it more rapid to find unknown genes.Based on sequence alignment in EST database,new chicken genes were looked for by in silico cloning.Finally a 947bp sequence was obtained from the EST,and named B8-t3.The sequencing results were reconsistent with our prediction,which validate the feasibility of in silico cloning.Protein analysis with B8-t3 was also made,the results showed B8-t3 codes for 206 amino acids and has 73%similarity with human muscle-specific beta 1 intergrin binding protein(MIBP).
Keywords:silico cloning  chicken  MIBP
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