| 引用本文: | 董 声,俞志明,宋秀贤,韩笑天,曹西华.类群特异性PCR引物设计与评估[J].生物信息学,2013,11(2):79-85. |
| DONG Sheng,YU Zhi-ming,SONG Xiu-xian,HAN Xiao-tian,CAO Xi-hua.Design and evaluation of group-targeted PCR primers[J].Chinese Journal of Bioinformatics,2013,11(2):79-85. |
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| 摘要: |
| 本文以真核藻类18S rDNA类群特异性PCR引物的设计与评估为例,详细介绍了如何利用Primrose等一系列程序设计类群特异性PCR引物并评估其敏感性与特异性,并对这一方法的优势与应用类群特异性PCR引物进行群落多样性分析需要注意的问题进行了讨论。 |
| 关键词: 类群特异性PCR引物 真核藻类 Primrose 18S rDNA通用引物 |
| DOI：10.3969/j.issn.1672-5565.2013-02.20130201 |
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| Design and evaluation of group-targeted PCR primers |
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DONG Sheng1,2,YU Zhi-ming1,SONG Xiu-xian1,HAN Xiao-tian1,CAO Xi-hua1
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(1.Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;;2.University of Chinese Academy of Sciences,Beijing 100049,China)
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| Abstract: |
| We take the design and evaluation of eukaryotic algae-targeted 18S rDNA PCR primers as an example, to give a step-by-step illustration of how to design and assess group-targeted PCR primers using Primrose and other programs. Then we summarize the advantages of the design & evaluation pipeline by comparing the amplification efficiency of newly designed and previously reported 18S rDNA universal primers. Cautions are also emphasized on using PCR-based strategies to assess community diversity. |
| Key words: Group-targeted Primers Eukaryotic Algae Primrose 18S rDNA Universal Primers |
