| 引用本文: | 陈哲伟,张萌,宋晓峰,赵健.内部核糖体进入位点(IRES)计算[]识别方法的研究进展[J].生物信息学,2025,23(4):249-260. |
| CHEN Zhewei,ZHANG Meng,SONG Xiaofeng,ZHAO Jian.Review of computational methods for predicting internal ribosomeentry site (IRES)[J].Chinese Journal of Bioinformatics,2025,23(4):249-260. |
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| 摘要: |
| 内部核糖体进入位点(Internal ribosome entry site,IRES)是一种RNA顺式作用元件,可以不依赖帽子结构启动翻译过程。其最早在病毒RNA中被报道,随后在真核生物的mRNA中也被发现。近来,IRES元件还被证实存在于环状RNA中,并介导其翻译。IRES元件介导的非帽依赖翻译起始机制在多个关键细胞生命活动过程中发挥着重要作用,并与多种人类复杂疾病的发生发展密切相关,引起越来越多研究者的关注。由于IRES元件的实验鉴定方法费时费力,因此,更为快速便捷的计算识别方法在前期实验数据基础上陆续被开发出来。本文对IRES元件进行了简要概述,总结了目前已知的IRES元件计算识别方法,并且建立了独立测试集对其预测性能进行了深入比较。 |
| 关键词: 内部核糖体进入位点 非帽依赖 翻译调控 机器学习 深度学习 |
| DOI：10.12113/202408007 |
| 分类号:R318.04 |
| 文献标识码:A |
| 基金项目:国家自然科学基金面上项目(No. 62273175);江苏省重点研发计划项目(No. BE2022843);中央高校基本科研业务费资助(No. 1003-XAA23018). |
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| Review of computational methods for predicting internal ribosomeentry site (IRES) |
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CHEN Zhewei, ZHANG Meng, SONG Xiaofeng,ZHAO Jian
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(College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 211106,China)
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| Abstract: |
| Internal ribosome entry site (IRES) is a cis-regulatory element that can initiate translation in cap-independent manner. It was first discovered in viral RNA sequences, and then cellular mRNAs were found to contain IRESs. Recently, IRES elements have also been reported in circRNAs and mediate their translation. Cap-independent translation mediated by IRES plays important roles in many cellular processes and is involved in many human diseases, receiving more and more attentions. Since identifying IRES elements by experimental method is time-consuming and laborious, faster and more convenient computational methods have been developed based on experimentally validated data. In this paper, we briefly introduce IRES elements, summarize existing IRES predicting methods, and compare their prediction performance on an independent testing dataset. |
| Key words: Internal ribosome entry site Cap-independent translation Translation regulation Machine learning Deep learning |
