| 引用本文: | 苏文霞,吕晓桂,张强.组蛋白修饰对胚胎干细胞分化过程[]的调控机制[J].生物信息学,2024,22(2):134-139. |
| SU Wenxia,LuXiaogui,ZHANG Qiang.Study on the regulatory mechanism of histone modifications in the differentiation of embryonic stem cell[J].Chinese Journal of Bioinformatics,2024,22(2):134-139. |
|
| 摘要: |
| 选用人类胚胎干细胞系和由人类胚胎干细胞系分化来的神经干细胞系为研究对象,分析组蛋白修饰对胚胎干细胞分化过程的调控作用。得到了两种细胞系差异表达基因转录起始位点侧翼区域内八种组蛋白修饰的分布模式,以及组蛋白修饰功能簇。研究表明在两类细胞系中,八种组蛋白修饰谱分布模式一致,且呈现两种分布类型； H3K27ac,H3K4me3和H3K9ac组成的功能簇是保守的;H3K27me3,H3K36me3和H3K79me1组成的功能簇以及H3K9me3和H3K27me3组成的功能簇在胚胎干细胞向神经干细胞分化的过程中消失。结果揭示了组蛋白修饰对胚胎干细胞系向神经干细胞系分化过程的部分调控机制,为该分化过程分子调控机制的研究提供部分重要的理论基础。 |
| 关键词: 胚胎干细胞 神经干细胞 分化 组蛋白修饰 |
| DOI：10.12113/202210002 |
| 分类号:Q61 |
| 文献标识码:A |
| 基金项目:国家自然科学基金项目(No.62201299)；内蒙古自治区自然科学基金项目(No.2021BS06003)；内蒙古自治区高等学校科学研究项目(No.NJZY21473)；内蒙古自治区直属高校基本科研业务费项目(No.BR220505). |
|
| Study on the regulatory mechanism of histone modifications in the differentiation of embryonic stem cell |
|
SU Wenxia, LuXiaogui, ZHANG Qiang
|
|
(College of Science, Inner Mongolia Agriculture University, Hohhot 010018, China)
|
| Abstract: |
| In this paper, human embryonic stem cell line and its differentiated neural stem cell line are selected as the objects to study the regulatory effect of histone modifications on the differentiation of embryonic stem cells. The distribution patterns of 8 histone modifications and functional clusters of histone modifications are obtained in the flanking regions of transcriptional initiation sites of the differentially expressed genes in the two cell lines. The results show that in the two cell lines, the distribution patterns of 8 histone modification profiles are consistent and show two distribution types; The functional clusters compose of H3K27ac, H3K4me3 and H3K9ac are conservative, while the functional clusters compose of H3K27me3, H3K36me3 and H3K79me1 and the functional clusters composed of H3K9me3 and H3K27me3 disappear during the differentiation of embryonic stem cells into neural stem cells. This paper reveals the partial regulatory mechanism of histone modifications on the differentiation of embryonic stem cell lines into neural stem cell lines and provids some important theoretical basis for the study of molecular regulatory mechanism of this differentiation process. |
| Key words: Embryonic stem cell Neural stem cell Differentiation Histone modification |
