| 引用本文: | 蒲鹏,李国强,刘法涛,刘颖斌.基于NGS的染色质测序在肿瘤研究中的应用[J].生物信息学,2024,22(2):79-92. |
| PU Peng,LI Guoqiang,LIU Fatao,LIU Yingbin.Application of NGS-based chromatin sequencing in cancer research[J].Chinese Journal of Bioinformatics,2024,22(2):79-92. |
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| 摘要: |
| 染色质是真核生物细胞核内由核酸和蛋白质组成的复合结构,有着精密且复杂的三维结构。染色质除基本的DNA序列外,内部还存在着不同化学修饰,DNA-蛋白质相互作用,DNA-DNA相互作用和DNA-RNA相互作用,以上这些若发生改变都可能在肿瘤发生发展过程中起到至关重要的作用。通过不同的染色质测序方法,可以解析出这些改变,并进一步加深研究者对肿瘤形成机制的理解,最终应用于肿瘤的治疗。本文对常见的染色质测序技术部分原理和应用进行综述。 |
| 关键词: NGS 染色质 肿瘤 |
| DOI：10.12113/202210025 |
| 分类号:Q75 |
| 文献标识码:A |
| 基金项目:国家自然科学基金 (No.2,1). |
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| Application of NGS-based chromatin sequencing in cancer research |
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PU Peng, LI Guoqiang, LIU Fatao, LIU Yingbin
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(Department of Biliary-Pancreatic Surgery,Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai Cancer Institute,State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University, Shanghai 200127, China)
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| Abstract: |
| All eukaryotic genomes are organized into a nucleoprotein complex called chromatin, which have a sophisticated and intricate three-dimensional structure. In addition to the basic DNA sequence, there also exist DNA modifications, DNA-protein interactions, DNA-DNA interactions and DNA-RNA interactions in chromatin, all of which, if altered, may play a vital role in cancer initiation and development. Through different chromatin sequencing techniques, researchers are able to parse these alterations and enhance our understanding of tumorigenesis, which could ultimately be applied to the cancer therapy. In this paper, we review some experimental principles and applications of common chromatin sequencing techniques. |
| Key words: NGS Chromatin Cancer |
