| 引用本文: | 林慧,陈佳菁,王灿明,郑素琼,邱建龙.miR-449a在乳腺癌组织中的表达及生物信息学分析[J].生物信息学,2023,21(3):171-178. |
| LIN Hui,CHEN Jiajing,WANG Canming,ZHENG Suqiong,QIU Jianlong.Expression and bioinformatic analysis of miR-449a in breast cancer tissues[J].Chinese Journal of Bioinformatics,2023,21(3):171-178. |
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| 摘要: |
| 探讨miR-449a在乳腺癌组织中的表达及其在乳腺癌发生发展过程中的作用。利用实时荧光定量PCR检测83例乳腺癌和癌旁组织中miR-449a的相对表达量,发现miR-449a在乳腺癌组织中的表达水平高于癌旁组织,并与肿瘤组织学级别、大小、雌激素受体状态和孕激素受体状态有关(P＜0.05)。miR-449a在三阴性乳腺癌中的表达水平显著低于管腔型。使用Kaplan-Meier Plotter数据库进行生存分析,结果显示在三阴性乳腺癌中miR-449a低表达组总生存率显著低于高表达组,而在管腔B型乳腺癌中miR-449a高表达组总生存率显著降低(P＜0.05)。利用ENCORI数据库预测得到靶基因186个,通过metascape数据库进行富集分析,发现其功能涉及间充质细胞分化、细胞迁移、内分泌抵抗、粘附连接、肌动蛋白细胞骨架调节以及NOTCH、TGF-β、Wnt、PI3K-Akt等介导的信号通路。通过string数据库进行蛋白互作网络分析,并使用Cytoscape软件筛选出由NOTCH1、JAG1和cyclin D1等蛋白构成的关键子网络。应用ENCORI数据库分析miR-449a与NOTCH途径靶基因的相关性,发现miR-449a与NOTCH1在乳腺癌组织中的表达呈负相关。本研究结果表明miR-449a在乳腺癌组织中的表达具有明显的异质性,可通过影响多种信号通路参与肿瘤发展过程,调控NOTCH信号通路可能是其在乳腺癌中的重要机制。 |
| 关键词: miR-449a 乳腺癌 靶基因 生物信息学 |
| DOI：10.12113/202209009 |
| 分类号:R737.9 |
| 文献标识码:A |
| 基金项目: |
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| Expression and bioinformatic analysis of miR-449a in breast cancer tissues |
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LIN Hui, CHEN Jiajing, WANG Canming, ZHENG Suqiong, QIU Jianlong
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(Department of Pathology, the 910th Hospital of the Chinese People's Liberation Army Joint Logistic Support Force, Quanzhou 362000, Fujian, China)
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| Abstract: |
| This study investigated the expression of miR-449a in breast cancer tissues and its role in the development of breast cancer. The relative expression of miR-449a in 83 cases of breast cancer tissues and adjacent tissues was detected using real-time fluorescent quantitative PCR. It was found that the expression level of miR-449a in breast cancer tissues was higher than that in adjacent tissues and was related to tumor histological grade, size, estrogen receptor status and progesterone receptor status (P<0.05). The expression level of miR-449a in triple negative breast cancer tissues was significantly lower than that in luminal breast cancer tissues. The Kaplan-Meier Plotter database was used for survival analysis. The results showed that the overall survival rate of the low expression group of miR-449a in triple negative breast cancer was significantly lower than that of the high expression group, while the overall survival rate of the high expression group of miR-449a in luminal B breast cancer was significantly lower (P<0.05). A total of 186 target genes were predicted via the ENCORI database and were enrichment-analyzed using the Metascape database. It was found that their functions involved mesenchymal cell differentiation, cell migration, endocrine resistance, adhesion, actin cytoskeleton regulation and signal transduction mediated by NOTCH, TGF-β, Wnt, PI3K-Akt and others. The protein interaction network was analyzed using the String database, and the key sub networks composed of NOTCH1, JAG1 and cyclin D1 proteins were screened using the Cytoscape software. The correlation between miR-449a and target genes of NOTCH pathway was analyzed using the ENCORI database, and the expression of miR-449a was negatively correlated with NOTCH1 in breast cancer tissues. This study demonstrates that the expression of miR-449a in breast cancer tissues is significantly heterogeneous, and it can participate in the tumor development process by affecting various signal pathways. Regulation of NOTCH signal pathway may be an important mechanism of miR-449a in breast cancer. |
| Key words: miR-449a Breast cancer Target gene Bioinformatics |
