| 引用本文: | 葛永飞,骆洁雅,叶子,林文娅,黄劲.基于生物信息学方法分析人CREB结合蛋白的结构与功能[J].生物信息学,2023,21(2):121-127. |
| GE Yongfei,LUO Jieya,YE Zi,LIN Wenya,HUANG Jin.Analysis of structure and function of human CREB-binding protein based on bioinformatics[J].Chinese Journal of Bioinformatics,2023,21(2):121-127. |
|
| 摘要: |
| 为了预测分析人CREB结合蛋白(CREB-binding protein,CBP)的结构和功能。本研究利用生物信息学相关数据库及软件对人CBP蛋白的理化性质、保守性、亚细胞定位、信号肽、跨膜结构域、二级结构、三级结构、相互作用蛋白及功能进行预测。结果表明,人CBP蛋白是一种定位于核内的不稳定亲水性蛋白质,无跨膜区和信号肽。其二级结构以无规卷曲和α-螺旋为主,并且该蛋白质HAT结构域在各物种间高度保守,推测与其酶活性密切相关的氨基酸残基为Tyr1433、Leu1434、Asp1435、Arg1664。此外,人CBP蛋白能够与TP53、CREB1、NCAO3等多种转录因子或转录辅激活因子发生相互作用,主要参与转录调控、细胞分化、组织发育、信号转导及细胞凋亡等生物学过程。本研究为进一步研究CBP在恶性肿瘤发生发展中的作用机制提供了理论依据。 |
| 关键词: 人CBP蛋白 理化性质 结构 生物信息学 |
| DOI：10.12113/202203013 |
| 分类号:Q51 |
| 文献标识码:A |
| 基金项目:贵州省2017年大学生创新创业训练计划项目(No.DC201710660028). |
|
| Analysis of structure and function of human CREB-binding protein based on bioinformatics |
|
GE Yongfei,LUO Jieya,YE Zi,LIN Wenya,HUANG Jin
|
|
(School of Basic Medicine, Guizhou Medical University, Guiyang 550025, China)
|
| Abstract: |
| In order to predict and analyze the structure and function of human CREB-binding protein (CBP), bioinformatics methods were used to predict the physical and chemical properties, conservation, subcellular localization, signal peptide, transmembrane domain, secondary structure, tertiary structure, interacting protein, and function of human CBP. Results showed that human CBP was an unstable and hydrophilic protein located in the nucleus without transmembrane region and signal peptide. The secondary structure was characterized by random coil and α-helix. The HAT domain of the protein was highly conserved among species. It was speculated that the amino acid residues closely related to its enzyme activity were Tyr1433, Leu1434, Asp1435, and Arg1664. In addition, human CBP could interact with transcription factors and transcription coactivators, such as TP53, CREB1, and NCAO3, and was mainly involved in biological processes such as transcription regulation, cell differentiation, tissue development, signal transduction, and apoptosis. This study provides a theoretical basis for further studying the mechanism of CBP in the occurrence and development of malignant tumors. |
| Key words: Human CBP Physicochemical properties Structure Bioinformatics |
