| 引用本文: | 王育选,张梦超,常丽君,张艾英,张莉,郭世华.谷子 MADS-box基因家族的鉴定和表达分析[J].生物信息学,2023,21(1):51-59. |
| WANG Yuxuan,ZHANG Mengchao,CHANG Lijun,ZHANG Aiying,ZHANG Li,GUO Shihua.Genome-wide identification and expression analysis of MADS-box genes in foxtail millet[J].Chinese Journal of Bioinformatics,2023,21(1):51-59. |
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| 摘要: |
| MADS-box基因是真核生物中一类重要的转录因子,参与调控多项植物的生长发育过程。然而关于谷子穗发育的MADS-box基因研究比较少。本研究使用序列相似性检索,在Phytozome 13.0数据库中筛选并且鉴定出了68个谷子MADS家族成员,并对这些家族成员的物理化学性质、系统发育树、染色体定位、表达谱等进行了全面的分析。结果表明,谷子MADS家族成员在染色体上分布不均匀,可以分为5个亚族。通过组织特异性表达谱分析得到,多数MADS基因在穗中表达量要高于其他器官。此外利用转录组测序技术对发育初期的谷穗和成熟期的谷穗进行了转录组测序分析,筛选到数个与谷穗分生组织发育相关MADS-box基因。为进一步揭示MADS-box基因在谷子穗发育过程中的作用奠定了重要的基础。 |
| 关键词: 谷子 MADS-box 表达谱 花序发育 |
| DOI：10.12113/202106006 |
| 分类号:Q311 |
| 文献标识码:A |
| 基金项目:国家自然科学基金(No.9,9)；山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目(No.202105D121008-2-5)；国家重点研发项目(No.2020YFD1000803-2)；山西省重点研发计划项目(No.201803D221019-1)；山西省应用基础研究计划项目(No.201901D111221)；山西农业大学生物育种工程项目(No.YZGC029). |
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| Genome-wide identification and expression analysis of MADS-box genes in foxtail millet |
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WANG Yuxuan1, ZHANG Mengchao1, CHANG Lijun1, ZHANG Aiying2, ZHANG Li1, GUO Shihua3
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(1. College of Agriculture,Shanxi Agricultural University, Taigu 030801,Shanxi,China; 2. Millet Research Institute, Shanxi Academy of Agricultural Sciences, Changzhi 046011,Shanxi,China; 3. College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010019,China)
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| Abstract: |
| MADS-box gene is an important transcription factor in eukaryote, which is involved in regulating a number of plant growth and development processes. While there are few studies on MADS-box genes of foxtail millet during panicle development. In this study, a genome-wide searching of foxtail millet MADS-box genes was performed in Phytozome 13.0 using sequence similarity search, and 68 foxtail millet MADS-box genes were screened and identified. The physical chemical property, phylogenetic tree, chromosome location, and expression profile of these family members were analyzed. Results showed that the members of the SiMADS family were divided into five subfamilies and unevenly distributed on chromosomes. According to tissue-specific expression patterns, the expression of most SiMADS genes in spike was higher than that in other tissues. Additionally, transcriptome analysis of spike in early stage and mature stage was conducted, and several spike differentiation related SiMADS genes were detected. The study provides important foundation for further revealing the function of MADS-box genes during foxtail millet inflorescence development. |
| Key words: Foxtail millet MADS-box Expression profile Inflorescence development |
