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主管单位 工业和信息化部 主办单位 哈尔滨工业大学 主编 任南琪 国际刊号ISSN 1672-5565 国内刊号CN 23-1513/Q

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引用本文:马尔合巴·艾司拜尔,成新琪,卢亚杰,李艳军,刘峰,秦江鸿,孙杰.陆地棉类谷氧还蛋(GhGRL)基因家族生物信息学分析[J].生物信息学,2021,19(2):105-114.
MAERHEBA.Aisibaie,CHENG Xinqi,LU Yajie,LI Yanjun,LIU Feng,QIN Jianghong,SUN Jie.Bioinformatics analysis of glutaredoxin-like protein (GhGRL) genes family in upland cotton[J].Chinese Journal of Bioinformatics,2021,19(2):105-114.
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陆地棉类谷氧还蛋(GhGRL)基因家族生物信息学分析
马尔合巴·艾司拜尔1,成新琪1,卢亚杰1,李艳军1,刘峰1,秦江鸿2,孙杰1
(1.石河子大学 农学院,新疆生产建设兵团绿洲生态农业重点实验室,新疆 石河子,832003; 2.石河子农业科学院 棉花研究所,新疆 石河子,832000)
摘要:
谷氧还蛋白(glutaredoxin,GRX)是一类小分子氧化还原蛋白,可以调节蛋白质的氧化还原状态从而维持蛋白质的功能,在生物的生长发育及抗氧化反应中起着重要的作用。类谷氧还蛋白蛋白(glutaredoxin-like,GRL)是新划分的GRX类型,本研究为深入探究GRL基因家族在陆地棉中功能,对GhGRL基因家族进行生物信息学及表达分析。研究结果表明,32 个GhGRL基因主要定位于细胞核,它们均具有GRX-GRX-Like保守结构域。GhGRL基因所编码的氨基酸的多重序列比对和保守序列分析发现,该家族成员序列相似性约为31.31%,大部分包含4个保守基序,同时这4个保守基序与保守结构域重叠;根据GhGRL基因的系统进化树可将32个GhGRL基因分为3亚组,基因结构分析发现该家族基因大部分无内含子;染色体定位分析显示GhGRL基因分散在19个染色体上,每条染色体上的GhGRL基因数目有很大的差别;表达谱数据分析表明,大部分GhGRL基因在根、茎、雄蕊、雌蕊、子房、叶片和花等7个组织器官中均有表达,并且有差异。以上结果有利于了解棉花GhGRL基因家族的基本情况,为深入研究该基因家族在生物学功能提供基础。
关键词:  陆地棉  类谷氧还蛋白基因  生物信息学  表达谱
DOI:10.12113/202005004
分类号:Q343.1
文献标识码:A
基金项目:国家自然科学基金项目(No.31860395).
Bioinformatics analysis of glutaredoxin-like protein (GhGRL) genes family in upland cotton
MAERHEBA Aisibaier1, CHENG Xinqi1, LU Yajie1, LI Yanjun1, LIU Feng1, QIN Jianghong2, SUN Jie1
(1.Key Laboratory of Oasis Ecological Agriculture, Xinjiang Production and Construction Crops,College of Agronomy, Shihezi University, Shihezi 832003, Xinjiang,China; 2.Cotton Research Institute, Shihezi Academy of Agricultural Sciences, Shihezi 832000, Xinjiang,China)
Abstract:
Glutaredoxin (gluxedoxin, GRX) is a type of small-molecule redox protein, which can regulate the redox balance of the protein to maintain the activity of the protein. It plays an important role in the growth and development and antioxidant response of many organisms. Glutaredoxin-like (GRL) is a newly classified type. In this study, bioinformatics and expression analysis of GhGRLgene family was conducted to explore the function of GRL gene family in Gossypium hirsutum. Results show that 32 GhGRLgenes were mainly located in the nucleus, all of which had GRX-GRX-like conserved domains. Multiple sequence alignment and conserved sequence analysis of the amino acids encoded by the GhGRLgene show that the family members had a sequence similarity of approximately 31.31% and most of the amino acid sequences encoded by GhGRLgene contained four conserved motifs, while these four conserved motifs overlapped with conserved domains. Phylogenetic tree and gene structure analysis of GhGRLgenes show that 32 GhGRL genes were divided into three subgroups, and most genes had no introns. Chromosome mapping analysis shows that 32 genes were scattered on 19 chromosomes and the number of GhGRLgenes on each chromosome was very different. Analysis of expression profile data shows that the GhGRLgenes were expressed in seven tissue organs, such as roots, stems, stamens, pistil, ovary, leaves, and flowers. The results of this study are helpful for understanding the evolution of the cotton GhGRLgene family and provide basis for further study on the biological function of this gene family.
Key words:  Gossypium hirsutum  Glutaredoxin-like protein  Bioinformatics  Expression profile

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