| 引用本文: | 曹英豪,彭公信.MutPrimerDesign:用于人类基因编码区域突变位点的引物设计程序[J].生物信息学,2020,18(3):169-175. |
| CAO Yinghao,PENG Gongxin.MutPrimerDesign: Design primers for human gene mutations located in coding sequence region[J].Chinese Journal of Bioinformatics,2020,18(3):169-175. |
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| 摘要: |
| 位于基因编码区的DNA突变与基因的功能密切相关。在已知人类基因编码区的突变位点时,如何在基因组上设计引物验证该突变是一个重要的问题。本文利用Python语言开发了引物设计程序MutPrimerDesign。MutPrimerDesign通过解析人类基因组序列数据库以及基因注释信息,转换基因编码区坐标为基因组坐标,并调用Primer3的python程序包接口,可批量自动化完成基因突变位点的引物及探针序列设计。MutPrimerDesign使用简便,可识别多种数据库的基因名称,并能够修改引物常规参数,实现引物的快速调整。 |
| 关键词: 引物设计 突变 生物信息学分析 |
| DOI：10.12113/201912004 |
| 分类号:Q344+.12 |
| 文献标识码:A |
| 基金项目:中国医学科学院医学与健康科技创新工程(No.2017-I2M-1-009). |
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| MutPrimerDesign: Design primers for human gene mutations located in coding sequence region |
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CAO Yinghao, PENG Gongxin
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(Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & School of Basic Medicine, Peking Union Medical College, Beijing 100730, China)
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| Abstract: |
| DNA mutations located in the coding region of a gene are closely related to the function of the gene. When the mutation site of the human gene protein coding region is known, how to design primers on the genome to validate the mutation becomes an important issue. In this study, MutPrimerDesign was developed as a primer design program by using Python language. Through analyzing human genome sequence and gene annotation information, MutPrimerDesign converts the gene coding region coordinates into genomic coordinates and calls the Python package interface of Primer3, which can automatically complete the primer and probe sequence design for the gene mutation in batches. MutPrimerDesign is easy to use, can recognize gene names in various databases, and can modify the general parameters of primers, thus achieving rapid adjustment of primers. |
| Key words: Primer design Mutation Bioinformatics analysis |
