| 引用本文: | 李惠敏,李璐璐,陈玉梅,李佳慧.罗汉果几丁质酶基因家族的生物信息学分析[J].生物信息学,2019,17(3):167-174. |
| LI Huimin,LI Lulu,CHEN Yumei,LI Jiahui.Bioinformatics analysis of chitinase gene family in Siraitia grosvenorii[J].Chinese Journal of Bioinformatics,2019,17(3):167-174. |
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| 摘要: |
| 几丁质酶是一类在植物抵抗病原真菌等过程中具有重要作用的蛋白质,为探讨几丁质酶在罗汉果抗根结线虫病中的调控作用,本研究基于南方根结线虫侵染下的罗汉果幼苗根系的转录组测序结果,采用生物信息学技术对筛选到的15个罗汉果几丁质酶基因进行分析。结果表明,15个罗汉果几丁质相关蛋白基因编码的氨基酸序列其N段均有一段信号肽,亚细胞定位在胞外；分子量从27 kDa到37 KDa不等；多数为酸性蛋白。基于氨基酸保守结构域和系统发育关系分析,15个罗汉果几丁质酶分属于GH18和GH19两大家族中的3个组别(I、III和IV)的成员,GH18家族成员三级结构预测具有典型的(α/β)8桶状结构,而GH19家族成员三级结构预测只有α螺旋结构域。这些分析结果可为今后深入研究罗汉果几丁质酶的生物学功能和调控机制提供一定的理论依据,为罗汉果抗根结线虫病育种提供参考。 |
| 关键词: 罗汉果 转录组测序 几丁质酶基因家族 理化特性 |
| DOI：10.12113/j.issn.1672-5565.201903006 |
| 分类号:S435.79 |
| 文献标识码:A |
| 基金项目: |
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| Bioinformatics analysis of chitinase gene family in Siraitia grosvenorii |
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LI Huimin, LI Lulu, CHEN Yumei, LI Jiahui
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(College of Life Science, Guangxi Normal University, Guilin 541006, Guangxi China)
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| Abstract: |
| Chitinases are proteins that play an important role in resistance to fungal diseases. To investigate the role of chitinases in the resistance of Siraitia grosvenoriito root-knot nematode (Meloidogyne incognita), 15 chitinase genes were analyzed by bioinformatics based on transcriptome analysis of Siraitia grosvenoriiseedlings infected by Meloidogyne incognita.Results showed that there was a signal peptide in the N-terminal of the amino acid sequences encoded by the 15 chitinase genes, and the subcellular localization was extracellular. The molecular weight ranged from 27 kDa to 37 KDa, and most of them were acidic proteins. The phylogenetic analysis based on amino acid conserved domain revealed that chitinases belong to three groups (I, III, and IV) of GH18 and GH19 families. The predicted tertiary structure of GH18 family members had a typical(α/β)8-barrel structure. However, there were only α-helix domains in the prediction of the tertiary structure of GH19 family members. These results would provide a reference for further study on the regulatory role of chitinase in the resistance mechanism of Siraitia grosvenoriito the root-knot in the future. |
| Key words: Siraitia grosvenorii RNA-seq Chitinase genes family Physical and chemical properity |
