| 引用本文: | 邹小龙,董雪松,孙学溥.结肠癌中核内miRNA的激活调控作用研究[J].生物信息学,2019,17(2):111-115. |
| ZOU Xiaolong,DONG Xuesong,SUN Xuepu.Activation regulation of nuclear miRNA regulation in colon cancer[J].Chinese Journal of Bioinformatics,2019,17(2):111-115. |
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| 摘要: |
| 为了探究增强子介导的核内miRNA在结肠癌发生中的作用,本研究筛选了结肠癌中的差异表达的miRNA数据、结肠的特异性增强子数据、结肠癌中差异表达基因数据,利用细胞核内miRNA靶向增强子预测算法,筛选miRNA调控的结肠特异性增强子；利用增强子靶基因预测数据,筛选核内miRNA调控的差异表达靶基因,并且构建核内miRNA-靶基因网络,并通过网络的分析和筛选获得结肠癌中关键的致病基因,同时对网络中的靶基因进行GO的功能注释。结果表明,我们所构建的核内miRNA-激活调控靶基因网络包含miRNA-靶基因关系对2 121个,259个节点,其中包含34个下调基因、183个上调的基因,7个下调的miRNA,35个上调的miRNA。而后我们分析了网络进行的节点度的整体分布情况,发现网络中大部分的节点的度都是小于10的,仅有少量miRNA结合和部分的差异表达基因节点的度大于10。核内miRNA主要通过激活调控了一些应激反应相关的功能和,同时,抑制调控了细胞周期、细胞凋亡、细胞死亡巨噬细胞代谢等相关功能,通过激活和抑制相关功能诱发结肠癌的发生。从核内miRNA的激活调控角度研究结肠癌的发病机制,是对原有细胞浆中miRNA抑制调控机制的补充,也为结肠癌的系统研究提供了新的视野。 |
| 关键词: 结肠癌 miRNA-增强子-靶基因网络 核内miRNA |
| DOI：10.12113/j.issn.1672-5565.201903009 |
| 分类号:Q522 |
| 文献标识码:A |
| 基金项目: |
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| Activation regulation of nuclear miRNA regulation in colon cancer |
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ZOU Xiaolong,DONG Xuesong, SUN Xuepu
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(Department of General Surgery, The First Affiliated Hospital of Harbin Medical University Heilongjiang, Harbin 150001,China)
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| Abstract: |
| To explore the role of enhancer-triggered nuclear miRNAs in colon cancer, this study selects the differentially expressed miRNA data, the colon specific enhancer data, and the differentially expressed gene data in colon cancer. The colon specific enhancers were screened using miRNA targeting enhancer prediction algorithm. Together with enhancer and target gene data, the nuclear miRNA triggered genes were filtered. The nuclear miRNA-target gene network was constructed by integrating all the data above. Topological properties of the network were analyzed, and the key pathogenic genes were found in the network. Finally, Gene Ontology annotation was conducted to all the target genes. Results showed that the miRNA-activated gene network contained 2 121 miRNA-target gene pairs and 259 nodes in total, including 34 down-regulated genes, 183 up-regulated genes, 7 down-regulated miRNAs, and 35 up-regulated miRNAs. Then we found that most of the nodes degrees in the network were less than 10, and only a small group of miRNAs and some of the differentially expressed gene nodes had higher degrees. Therefore, nuclear miRNAs mainly regulated some stress-related functions through activating gene expression. At the same time, they negatively regulated the cell cycle, apoptosis, cell death, macrophage metabolism, and other related functions. Study on the pathogenesis of colon cancer from the activation regulation of nucleus miRNAs is a supplement to its negative regulation mechanism in cell cytoplasm, and provides a new insight for the systematic study in colon cancer. |
| Key words: Colon cancer miRNA-enhancer-gene network Nuclear miRNA |
