| 引用本文: | 李贝贝,李梦臻,张芃芃,管希云.基于纳米孔通道检测microRNAs对乳腺癌辅助诊断的研究[J].生物信息学,2018,16(4):269-275. |
| LI Beibei,LI Mengzhen,ZHANG Pengpeng,GUAN Xiyun.Research on the auxiliary diagnosis of breast cancer based on the detection of microRNAs by nanopore channel detection[J].Chinese Journal of Bioinformatics,2018,16(4):269-275. |
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| 摘要: |
| 乳腺癌的发生、发展与miRNA 31、miRNA 195、 miRNA 892b 等的异常表达明显相关。因此,miRNA 31、miRNA 195、 miRNA 892b可作为乳腺癌的生物标志物及治疗预后指标。本文研究了一种对乳腺癌早期检测的新方法:设计探针(probe)并使用α-溶血素(αHL)纳米孔道单分子检测方法检测乳腺癌miRNAs。DNA 探针与 miRNA的中间序列完全匹配,特异性识别目标miRNA。miRNA·probe 复合物分子穿过纳米孔道时, 由于probe 序列不同导致复合物分子与αHL 相互作用不同, 以至于miRNA 31·probe 31、miRNA 195·probe 195、miRNA 892b·probe 892b 有不同形状和不同堵塞时间特征信号, 可以有效区分该三种miRNAs。因此,未来有望实现乳腺癌的早期检测。 |
| 关键词: microRNA 早期诊断 乳腺癌 α-溶血素 |
| DOI：10.12113/j.issn.1672-5565.201804002 |
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| 文献标识码:A |
| 基金项目: |
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| Research on the auxiliary diagnosis of breast cancer based on the detection of microRNAs by nanopore channel detection |
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LI Beibei, LI Mengzhen, ZHANG Pengpeng, GUAN Xiyun
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(Henan Jintai Biological Technology Co., Ltd., Zhengzhou 450000,China)
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| Abstract: |
| The occurrence and development of breast cancer is clearly associated with abnormal expression of miRNA 31, miRNA 195, and miRNA 892b, which can be used as valuable biomarkers and prognostic indices for breast cancer. Herein, we report a new method for early detection of breast cancer, where an α-hemolysin (αHL) nanopore was utilized as a sensing element for the breast cancer-associated miRNAs analysis in the presence of selective DNA probes. The DNA probe was well-matched with the middle sequence segment of the target miRNA, offering specific recognition of the analyte miRNA. When miRNA·probe hybridization molecules passed through the nanopore, the difference in the probe sequences led to a significant difference in the interactions between the hybridization molecules and the αHL pore, so that miRNA 31·probe 31, miRNA 195·probe 195, and miRNA 892b·probe 892b produced different characteristic signals, thus achieving the effective distinguishment among the three miRNAs. It is expected that early breast cancer detection might be accomplished in the near future. |
| Key words: MicroRNA Early diagnosis Breast cancer αHL |
