| 引用本文: | 张云璇,屈丽娜,李新梅,刘德荣,赵小英.受脱落酸调节的拟南芥F-box基因的差异表达分析[J].生物信息学,2018,16(4):222-230. |
| ZHANG Yunxuan,QU Lina,LI Xinmei,LIU Derong,ZHAO Xiaoying.Differential expression analysis of F-box genes regulated by abscisic acid in Arabidopsis[J].Chinese Journal of Bioinformatics,2018,16(4):222-230. |
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| 摘要: |
| 脱落酸(Abscisic acid,ABA)是一种重要的植物激素,在种子休眠的建立、种子萌发、根发育和非生物胁迫反应过程中发挥作用。F-box蛋白是E3泛素连接酶SCF复合体的组成部分,通过特异识别和调节底物蛋白水平而调控植物生长发育过程。通过分析GEO基因芯片,筛选到38个受ABA调节的拟南芥候选F-box基因。选择其中6个F-box基因,进行实时荧光定量PCR分析。研究结果与基因芯片结果基本一致。分析启动子,发现候选基因含有大量ABA、干旱和胁迫相关的顺式作用元件。分析基因表达谱,发现部分基因在保卫细胞、种皮、花粉和衰老叶片中呈现高表达；大部分基因在ABA处理、胁迫和种子吸胀过程中表达量改变显著。这些分析结果为深入研究ABA调节植物生长发育和抗逆的分子机制提供了线索。 |
| 关键词: 脱落酸 F-box基因 拟南芥 基因芯片 |
| DOI：10.12113/j.issn.1672-5565.201803001 |
| 分类号:Q343.1 |
| 文献标识码:A |
| 基金项目:国家自然科学基金项目(No:31171176)；湖南省自然科学基金项目(No:11JJA002). |
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| Differential expression analysis of F-box genes regulated by abscisic acid in Arabidopsis |
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ZHANG Yunxuan, QU Lina, LI Xinmei, LIU Derong, ZHAO Xiaoying
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(Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation(College of Biology, Hunan University), Changsha 410082, China)
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| Abstract: |
| Abscisic acid (ABA) is an important phytohormone that plays a role in the establishment of seed dormancy, seed germination, root development, and abiotic stress responses. F-box protein is part of the E3 ligase in SCF complex, which regulates plant growth and development through specific recognition and regulation of substrate protein levels. By analyzing the GEO gene microarray, we screened 38 ABA-regulated Arabidopsiscandidate F-boxgenes and six of them were selected for real-time fluorescence quantitative PCR analysis. The results were consistent with those of the gene microarrays. Analysis of the promoter revealed that the candidate genes contained large amounts of ABA, drought, and stress-related cis-acting elements. Analysis of gene expression profiles revealed that some genes showed high expression in guard cells, seed coats, pollen, and senescent leaves; most of the genes showed significant changes in ABA processing, stress, and seed stratification. These findings provide clues for further study of the molecular mechanism of ABA regulation in plant growth, development, and stress resistance. |
| Key words: ABA F-boxgene Arabidopsis Gene microarray |
