| 摘要: |
| 针对RIPK4(Receptor-interacting serine/threonine kinase protein 4)结构与功能的报道较少且矛盾。本研究使用生物信息学手段,对RIPK4蛋白的理化性质、组织表达、亚细胞定位、信号肽和跨膜区、空间结构、蛋白质相互作用网络及序列同源性进行分析。结果表明人RIPK4蛋白是酸性不稳定的亲水蛋白,无信号肽和跨膜区域,定位于细胞质的可能性最大,主要二级结构为α-螺旋和无规则卷曲,属于PKc_like和ANK超家族。经GO分析和KEGG通路分析可知,与RIPK4相互作用的蛋白PHLPP1、PHLPP2、ACACA、ACACB、CNOT6L和CNOT6值得深入研究,预示RIPK4存在更为复杂的分子功能和作用机制。为进一步研究RIPK4的功能提供一定的参考。 |
| 关键词: RIPK4 生物信息学 结构 功能 相互作用蛋白 |
| DOI：10.3969/j.issn.1672-5565.201708006 |
| 分类号:Q71 |
| 文献标识码:A |
| 基金项目: |
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| Bioinformatics analysis of the structure and function of RIPK4 |
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LIU Chang1,2, LIU An3
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(1．Central Laboratory, Changzhi Medical College, Changzhi 046000, China; 2. Department of Biochemistry andMolecular Biology, Shanxi Medical University, Taiyuan 030001, China; 3 . Medical Department, Heji Hospital Affiliated to Changzhi Medical College, Changzhi 046000, China)
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| Abstract: |
| There are scant and contradictory reports on the structure and function of RIPK4 (receptor-interacting serine / threonine kinase protein 4). In this paper, bioinformatics methods were applied to analyze the chemical properties, tissue expression, subcellular localization, signal peptide, trans-membrane region, space structure, protein interaction networks and heredity conservation of RIPK4. The results show that the RIPK4 is hydrophilic protein with unstable acidity without signal peptide and trans-membrane region, which is most likely located in cytoplasm. Its main secondary structure elements are alpha helix and random coil, and it belongs to PKc_like and ANK superfamily. GO analysis and KEGG pathway analysis show that interactive proteins PHLPP1, PHLPP2, ACACA, ACACB, CNOT6L and CNOT6 deserve further study and indicate that RIPK4 has more complex molecular functions and mechanism of action. The study provides some reference for further study of the function of RIPK4 . |
| Key words: RIPK4 Bioinformatics Structure Function Interactive proteins |
