| 引用本文: | 范磊,刘雪飞,黄新河.多球壳菌素介导线粒体功能调控酵母细胞衰老[J].生物信息学,2018,16(2):90-96. |
| FAN Lei,LIU Xuefei,HUANG Xinhe.Myriocin-mediated mitochondrial function regulates cellular aging of Saccharomyces cerevisiae[J].Chinese Journal of Bioinformatics,2018,16(2):90-96. |
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| 摘要: |
| 多球壳菌素是前期鉴定出的一种抗衰老活性小分子,为了较全面理解多球壳菌素的抗衰机制,本研究利用模式生物芽殖酵母为材料,借助DNA Microarray技术、生物信息学手段并结合相关功能实验分析了多球壳菌素对对数期芽殖酵母细胞基因表达谱、基因本体聚类及相关信号通路的影响。结果显示,多球壳菌素对细胞转录组产生了显著影响,共导致1 648个基因的差异表达(FDR<0.05,Fold Change>1.5),其中843个基因显著上调,805个基因显著下调。进一步对基因本体聚类、信号通路分析及功能实验验证显示,线粒体相关功能和信号通路是多球壳菌素作用的一个主要靶点,本研究对多球壳菌素的进一步开发利用奠定了重要的理论基础。 |
| 关键词: 多球壳菌素 芽殖酵母 转录组 线粒体 抗衰老 |
| DOI：10.3969/j.issn.1672-5565.201802004 |
| 分类号:Q255 |
| 文献标识码:A |
| 基金项目:四川省科技厅应用基础研究项目(No. 2016JY0113)；成都市科学技术局科技惠民计划(No. 2015-HMO1-00047-SF)；中央高校科技创新项目(No. 2682016CX099)；中国大学生科研训练计划项目(No. 201710613066). |
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| Myriocin-mediated mitochondrial function regulates cellular aging of Saccharomyces cerevisiae |
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FAN Lei, LIU Xuefei, HUANG Xinhe
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(School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China)
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| Abstract: |
| Myriocin is a previously identified anti-aging active small molecule. In order to better understand its anti-aging mechanism, model organism Saccharomyces cerevisiaewas utilized as a model, and DNA Microarray, bioinformatics methods as well as related functional experiments were employed to analyze the influence of myrioncin on the gene expression profile, Gene Ontology (GO) clustering and related signaling pathways of Saccharomyces cerevisiaein log phase. Overall results showed that the myriocin produced a significant effect on the transcriptome of log phase yeast cells. It led to 1 648 differentially expressed genes (DEGs) (FDR<0.05, Fold Change>1.5), which included 843 up-regulated DEGs and 805 down-regulated DEGs. Further GO clustering, signaling pathway analysis, and functional experiments showed mitochondrial-related function and signaling pathways were the main targets of myriocin. This study provides important theoretical basis for further research and development of myriocin. |
| Key words: Myriocin Saccharomycescerevisiae Transcriptome Mitochondria Anti-aging |
