| 摘要: |
| 核糖体蛋白S6激酶β1(Ribosomal protein S6 kinase β1, RPS6KB1)是一个有价值的肝癌诊断和预后标志物,也是一个潜在的基因治疗分子靶点,但其致癌机制和预后价值仍未完全阐明。为了全面认识RPS6KB1,本文使用生物信息学手段,对RPS6KB1蛋白的序列同源性、组织表达、亚细胞定位、理化性质、空间结构及蛋白质相互作用网络进行分析。结果表明人RPS6KB1基因编码525个氨基酸组成的多肽,在进化过程中高度保守,属于PKc_like超家族,是酸性不稳定的亲水蛋白,无信号肽和跨膜区域。该蛋白定位于细胞核的可能性最大,主要二级结构为随机卷曲,存在磷酸化、乙酰化和泛素化位点。与RPS6KB1相互作用的蛋白主要是mTOR信号途径相关蛋白、PI3K信号途径相关蛋白、蛋白质合成相关蛋白以及调控胰岛素水平相关蛋白。本文结果为进一步研究RPS6KB1的功能及致癌机制提供一定的参考。 |
| 关键词: 核糖体蛋白S6激酶β1 肝癌 相互作用蛋白 预后标志物 功能 |
| DOI：10.3969/j.issn.1672-5565.201706004 |
| 分类号:Q71 |
| 文献标识码:A |
| 基金项目: |
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| Analysis of the molecular structure and physicochemical property ofribosomal protein S6 kinase β1 |
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LIU Chang1,2, LIU An3
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(1.Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China; 2.Central Laboratory of Changzhi Medical College, Changzhi 046000, China; 3. Medical Department, Heji Hospital Affiliated to Changzhi Medical College, Changzhi 046000, China)
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| Abstract: |
| Ribosomal protein S6 kinase β1(RPS6KB1)is a valuable diagnosis and prognostic marker for hepatocellular carcinoma and a potential target for gene therapy, but its carcinogenic mechanism and prognostic value have not been fully elucidated. To gain insightful information of RPS6KB1 protein, bioinformatics methods are applied to analyze the hereditary conservation, tissue expression, subcellular localization, chemical properties, space structure and protein interaction networks of RPS6KB1 protein. The RPS6KB1 protein is highly conserved and comprised of 525 amino acid residues and belongs to the PKc_like super family. It is a hydrophilic unstable protein without signal peptide and trans-membrane region. It is mainly located in the nucleus and the main secondary structure elements are random coil. It contains several phosphorylation, acetylation and ubiquitination sites. Interactive proteins with RPS6KB1 are mainly proteins associated with mTOR signal pathway, PI3K signal pathway, protein synthesis and insulin regulation. Our results provide some reference for further study on the function and carcinogenic mechanism of the protein. |
| Key words: RPS6KB1 Hepatocellular carcinoma Interactive proteins Prognostic marker Function |
