| 引用本文: | 沈成,童侨,金一然,袁辰阳,罗静帆,黄新河.两种药物协同效应对酵母细胞转录组的影响[J].生物信息学,2017,15(4):229-234. |
| SHEN Cheng,TONG Qiao,JIN Yiran,YUAN Chenyang,LUO Jingfan,HUANG Xinhe.Influence of synergy by two drugs on transcriptome of Saccharomyces cerevisiae[J].Chinese Journal of Bioinformatics,2017,15(4):229-234. |
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| 摘要: |
| 寻找抗衰老活性分子并研究其作用机制是衰老药物学的研究重点和热点。前期研究发现活性分子多球壳菌素和雷帕霉素可通过分子间协同效应延缓芽殖酵母细胞衰老。为了考察这两种小分子协同效应对细胞转录组的影响,利用DNA Microarray技术及生物信息学手段系统分析了这两种小分子药物协同处理对基因表达谱、基因本体聚类及相关信号通路的影响。研究结果显示,药物协同效应对细胞转录组产生了显著影响,共导致2 546个基因的差异表达(FDR < 0.05,Fold Change > 10%),其中1 157个基因显著上调,1 389个基因显著下调。进一步对基因本体聚类及信号通路分析显示,线粒体相关的分子功能、细胞组分、生物学过程和信号通路是此协同效应的一个主要靶点,其他可能的作用靶点还包括核糖体的生物发生、细胞骨架及过氧化物酶体代谢。 |
| 关键词: 多球壳菌素 雷帕霉素 协同效应 芽殖酵母 抗衰老 |
| DOI：10.3969/j.issn.1672-5565.201704002 |
| 分类号:Q255 |
| 文献标识码:A |
| 基金项目:中央高校科技创新项目(2682016CX099)；中国大学生科研训练计划项目(201610613063);四川省科技厅应用基础研究项目(2016JY0113)；成都市科学技术局科技惠民计划(2015-HMOJ-00047-SF). |
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| Influence of synergy by two drugs on transcriptome of Saccharomyces cerevisiae |
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SHEN Cheng, TONG Qiao, JIN Yiran, YUAN Chenyang, LUO Jingfan, HUANG Xinhe
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(School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China)
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| Abstract: |
| Searching for anti-aging compounds and studying its mechanism of action are the focus and hotspot of aging pharmacology. Previous studies have shown myriocin and rapamycin could delay the aging of Saccharomyces cerevisiaethrough intermolecular synergistic effects. To study the influence of synergistic effects of these two small molecules on transcriptome, DNA Microarray assay and bioinformatic analysis were used to analyze synergistic effects of these two drugs on gene expression, gene ontology and related signaling pathways as well. Overall results showed synergistic effects of myriocin and rapamycin produced a significant effect on the transcriptome, leading to 2546 differentially expressed genes (DEGs) (FDR < 0.05, Fold Change > 10%) including 1157 up-regulated DEGs and 1389 down-regulated DEGs. Further gene ontology clustering and signal pathway analysis showed mitochondrial-related cellular component, molecular function, biological processes and signaling pathways are one of the main targets of synergistic effects, and other possible targets include ribosome biogenesis, cytoskeletal organization, and peroxisome metabolisms. |
| Key words: Myriocin Rapamycin Synergistic effects Saccharomycescerevisiae Anti-aging |
