| 引用本文: | 刘红美,张洁,李玉权,陶小买,龚晓林.家蝇Prohibitin基因的生物信息学分析[J].生物信息学,2016,14(4):213-218. |
| LIU Hongmei,ZHANG Jie,LI Yuquan,TAO Xiaomai,GONG Xiaolin.Bioinformatics analysis of Prohibitingene of Muscadomestica (Housefly)[J].Chinese Journal of Bioinformatics,2016,14(4):213-218. |
|
| 摘要: |
| 为对家蝇Prohibitin蛋白序列进行生物信息学分析,从而为该基因功能研究奠定基础。利用在线分析程序和相关工具软件分析Prohibitin蛋白的理化性质、结构域、并预测其空间结构和功能。结果表明家蝇Prohibitin蛋白由277个氨基酸组成,分子量为30.54 kDa,理论等电点为5.26,为稳定蛋白,有跨膜区,但不含信号肽,该蛋白属于PHB保守结构域家族,亚细胞定位于细胞质,二级结构以α-螺旋为主。蛋白同源性比对结果显示,昆虫中的Prohibitin蛋白具有较高的同源性。这些分析结果可为今后深入研究该蛋白的结构特征和功能提供参考。 |
| 关键词: 家蝇 Prohibitin基因 生物信息学 进化树 |
| DOI：10.3969/j.issn.1672-5565.2016.04.03 |
| 分类号:Q343.1 |
| 文献标识码:A |
| 基金项目:贵州省高层次人才基金项目(No.876)。 * |
|
| Bioinformatics analysis of Prohibitingene of Muscadomestica (Housefly) |
|
LIU Hongmei 1, ZHANG Jie1 ,LI Yuquan1, TAO Xiaomai1 , GONG Xiaolin2
|
|
(1.College of Biology and Engineering,Guizhou Medical University, Guiyang 550004,China; 2.Department of Technology of Library, Guizhou Medical University, Guiyang 550004,China)
|
| Abstract: |
| To perform the bioinformatics analysis of Prohibitingene of Muscadomestica(Housefly),and aims at providing the basis for its’ function research. Internet online procedures and the related software are exploited to analyze the physical and chemical properties of Prohibitin protein and the domains of the protein, and predict the structure and functions of the protein. The Prohibitin protein sequence is composed of 277amino acids with 30.54 kDa of the molecular weight. Theoretical isoelectric point is 5.26. The protein is stable and had structures of transmembrane and helical coils. The protein belongs to the family of PHB,and is located in cytoplasm and dominated by alpha helix in second structure. The phylogenetic tree reveals that the Prohibitin protein in insect has the highest homology. This work provides a systemic sequence analysis of Prohibitin protein of Muscadomestica(Housefly). It will provide the useful reference for further investigation of its structure and function. |
| Key words: Muscadomestica Prohibitingene Bioinformatics Phylogenetic tree |
