| 引用本文: | 张鹏飞,王宁,周蔓,李萍.牦牛儿基牻牛儿基焦磷酸合成酶(GGPS)的生物信息学分析[J].生物信息学,2016,14(3):127-133. |
| ZHANG Pengfei,WANG Ning,ZHOU Man,LI Ping.Bioinformatics analysis of geranylgeranyl pyrophosphate synthase in plants[J].Chinese Journal of Bioinformatics,2016,14(3):127-133. |
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| 摘要: |
| GGPS是萜类化合物合成的一个重要分支点酶,同时也是合成GGPP的催化酶。本课题共选择了9个不同科的植物,对其GGPS核苷酸及氨基酸序列的理化性质、蛋白质结构功能以及亲缘进化关系等进行详细的分析。结果表明,九个科植物GGPS核苷酸序列长度均大于1 000 bp小于2 000 bp；GGPS氨基酸都不含有信号肽,不存在跨膜结构域；GGPS蛋白的二级结构中α螺旋和无规卷曲是主要结构,且无β-折叠结构,延伸链和β-转角则分散于整个蛋白中。通过生物信息学方法进行合理预测,为进一步研究GGPS的酶学特性提供了重要理论依据。 |
| 关键词: 生物信息学 GGPS 分子进化 |
| DOI：10.3969/j.issn.1672-5565.2016.03.01 |
| 分类号:Q55 |
| 文献标识码:A |
| 基金项目: |
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| Bioinformatics analysis of geranylgeranyl pyrophosphate synthase in plants |
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ZHANG Pengfei,WANG Ning,ZHOU Man,LI Ping
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(School of Life Science and Engineering, Southwest Jiaotong University,Chengdu 610031,China)
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| Abstract: |
| GGPS is an important branch point enzyme for terpenoids systhesis, but also its catalyting enzyme of GGPS.This study select 9 different families of plants, implement a series of detailed analyses to the physicochemical properties of GGPS nucleotide and amino acid sequence,protein structure and function and phylogenetic and so on. The results indicate that full length of DNA sequence from 9 species is in the range from 1 000 bp to 2 000 bp. All the GGPS amino acid sequence dont contain signal peptide and trmenbrance structure; α-helix and random coil are the primary structure of GGPS secondary structure and dont have any β-sheet structure, extension chain and β-corner are scattered throughout the protein.Reasonably prediction based on bioinformatics methods provide important theoretical basis for further research GGPS enzymatic properties. |
| Key words: Bioinformatics GGPS Molecular evolution |
