| 引用本文: | 王顺利,庞瑞华,黄英,李永能,李琦华,潘洪彬,杨明华,赵素梅.鸡Sepp1基因及其蛋白理化性质和分子结构的生物信息学分析[J].生物信息学,2016,14(2):84-94. |
| WANG Shunli,PANG Ruihua,HUANG Ying,LI Yongneng,LI Qihua,PAN Hongbin,YANG Minghua,ZHAO Sumei.Bioformatic analysis of chicken Sepp1 gene and its protein property and ptructure[J].Chinese Journal of Bioinformatics,2016,14(2):84-94. |
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| 鸡Sepp1基因及其蛋白理化性质和分子结构的生物信息学分析 |
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王顺利1,庞瑞华2,黄英1,李永能3,李琦华1,潘洪彬1,杨明华1,赵素梅1
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(1.云南农业大学云南省动物营养与饲料重点实验室,昆明 650201; (2.河南信阳师范学院生命科学学院,河南 信阳 464000; (3.云南农业大学招生就业处,昆明 650201)
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| 摘要: |
| 硒是人和动物生命必需的微量元素,硒的生物学功能是通过硒蛋白来实现的,硒蛋白P(Selenoprotein P,Sepp1)是机体含量最多的硒蛋白。利用生物信息学相关分析软件和比较基因组学的方法,对鸡Sepp[ST5"BX]1基因序列和氨基酸序列进行理化性质和结构特征进行分析和预测,并与猪、人、鼠、羊、狗等物种进行比较。结果显示,鸡Sepp[ST5"BX]1的CDS序列和编码的蛋白质与禽类动物相似,但和哺乳类动物之间差别较大；Sepp1的功能特性与硒半胱氨酸含量有关,不同物种Sepp1中硒半胱氨酸含量存在着差异；鸡Sepp1蛋白在第170-200位氨基酸残基区域具有特定理化性质和分子结构,推测该序列可作为调控鸡Sepp1功能的靶位点。研究结果为以后深入研究鸡Sepp1的机体功能、开发富集Se的动物功能性食品提供理论参考。 |
| 关键词: Sepp1 鸡 生物信息学 理化性质 分子结构 |
| DOI：10.3969/j.issn.1672-5565.2016.02.04 |
| 分类号:Q343.3+1 |
| 文献标识码:A |
| 基金项目:国家高技术研究发展计划项目(No.2011AA100305)。 |
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| Bioformatic analysis of chicken Sepp1 gene and its protein property and ptructure |
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WANG Shunli1,PANG Ruihua2,HUANG Ying1,LI Yongneng3,LI Qihua1, PAN Hongbin1,YANG Minghua1,ZHAO Sumei1
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(1．Yunnan Key Lab of Agricultural Animal Nutrition and Feed Science,Yunnan Agricultural University,Kunming 650201,China; (2． College of Life Science, HeNan Xinyang Normal University, Xinyang Henan 464000,China; (3．Admission and Employment Office, Yunnan Agricultural University, Kunming 650201,China)
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| Abstract: |
| Selenium is the life essential trace elements for human and animal. The biological function of selenium is exerted through selenoproteins. Selenoprotein P (selenoprotein P, sepp1) is the maximum content of selenoprotein in body. In this study, the characteristics of chicken Sepp1 gene sequence and amino acid sequence were analyzed and predicted as well as were compared with that of pig, human, rat, sheep, dog and other species by bioinformatics analysis software and comparative genomics method. The results showed the similar chicken Sepp1 CDs sequence and coding protein among poultry animals, but big difference among mammalian animals. Chicken Sepp1 functional properties were related to Sec content. Variant Sec of Sepp1 content exist difference among species. The 170 to 200 amino acid residues region of chicken sepp1 protein with specific physicochemical properties and molecular structure suggests that the sequence could be regarded as the target regulation site of selenoprotein P. The results would provide theoretical basis for further study on body functions of chicken selenoprotein P and development the functional animal food of Se enrichment. |
| Key words: Sepp1 Chicken Bioinformatics Physicochemical properties Molecular structure |
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