| 引用本文: | 张燕,王玉彩,刘振东,赵颖馨,张华.流体剪切力对内皮细胞miR-21和miR-199a表达的影响[J].生物信息学,2016,14(1):19-25. |
| ZHANG Yan,WANG Yucai,LIU Zhendong,ZHAO Yingxin,ZHANG Hua.Effect of fluid shear stress on expression of miR-21 and miR-199a in endothelial cells[J].Chinese Journal of Bioinformatics,2016,14(1):19-25. |
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| 流体剪切力对内皮细胞miR-21和miR-199a表达的影响 |
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张燕1,2,3,王玉彩3,刘振东1,赵颖馨1,张华1
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(1．山东省医学科学院基础医学研究所, 济南 250062;2.济南大学,山东省医学科学院医学与生命科学学院, 济南 250022;3.山东省齐河县人民医院,山东齐河 251100)
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| 摘要: |
| 为探讨流体剪切力对内皮细胞micorRNAs表达的影响。采用旋转锥形圆盘剪切力系统对内皮细胞分别加载低(4 dyn/cm2)、中(10 dyn/cm2)和高(15 dyn/cm2)3种不同梯度的剪切力作用24h。对照组未加载剪切力。采用高通量筛选芯片检测microRNAs表达变化,qRT-PCR验证,并进行生物信息学分析。与对照组比较,低剪切力组表达差异的microRNAs有33个(FC＞1.5或＜0.5倍,P＜0.05),其中28个上调,5个下调；中剪切力组表达差异的microRNAs有8个(FC＞1.5或＜0.5倍,P＜0.05),其中6个上调,2个下调；高剪切力组表达差异的microRNAs有31个(FC＞1.5或＜0.5倍,P＜0.05),其中25个上调,6个下调。miR-21在高剪切力组中上调最显著(FC = 0.026), 在低剪切力组中显著下调(FC = 3.531)。miR-199a在低剪切力组中上调最显著(FC = 0.075),在高剪切力组中显著下调(FC = 3.031)。表达差异的microRNA的靶基因主要与内皮细胞的力学信号转导、细胞跨膜迁移、钙离子信号通路、细胞内吞作用等相关。流体剪切力可诱导内皮细胞miR-21和miR-199a表达发生改变。 |
| 关键词: 剪切力 内皮细胞 microRNA 表达 |
| DOI：10.3969/j.issn.1672-5565.2016.01.04 |
| 分类号:Q344+.13 |
| 文献标识码:A |
| 基金项目:国家自然科学基金项目(81470489);山东省自然科学基金项目(ZR2014HM098,)；山东省医药卫生科技发展计划项目(2014WS2,4WS0316)。 |
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| Effect of fluid shear stress on expression of miR-21 and miR-199a in endothelial cells |
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ZHANG Yan1,2,3, WANG Yucai3, LIU Zhendong1, ZHAO Yingxin1,ZHANG Hua1
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(1.Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China; 2.School of Medicine and Life Sciences, University of Jinan ＆ Shandong Academy of Medical Sciences, Jinan 250022, China; 3.People’s Hospital of Qihe, Qihe Shandong 251100, China)
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| Abstract: |
| To evaluate the effect of fluid shear stress on expression of microRNAs in endothelial cells.Low (4 dyn/cm2), middle (10 dyn/cm2), and high (15 dyn/cm2) fluid shear stress were loaded onto endothelial cells for 24 h using rotating cone disc shear stress system, respectively. No shear stress was loaded onto endothelial cells in control group. Changes of microRNAs expression were assessed using high throughput screening chip. The results were verified using quantitative real-time polymerase chain reaction (qRT-PCR). Bioinformatics analysis was performed in difference-expressed microRNAs.Compared to control group, there were 33 differentially expressed microRNAs in low shear stress group. Among them, 28 microRNAs expression were up-regulated and 5 microRNAs expression were down-regulated. In middle shear stress group, there were 8 differentially expressed microRNAs compared to control group. Among them, 6 microRNAs expression were up-regulated and 2 microRNAs expression were down-regulated. In high shear stress group, there were 31 microRNAs expression changed compared to control group. Among them, 25 microRNAs expression were up-regulated and 6 microRNAs expression were down-regulated.MiR-21 was markedly up-regulated in high shear stress group (fold change: 0.026) and significantly down-regulated in low shear stress group (fold change:3.531). MiR-199a was markedly up-regulated in low shear stress group (fold change: 0.075) and significantly down-regulated in high shear stress group (fold change:3.031). The results of bioinformatics analysis showed that target genes of differentially expressed microRNAs related to mechanical signal transduction, cell trans membrane transport, calcium ion signaling pathway, and endocytosis of cells.The change of the expressions of miR-21 and miR-199a were induced by fluid shear stress in endothelial cells. |
| Key words: Shear stress Endothelial cell MicroRNA Expression |
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