| 摘要: |
| 为探讨microRNA-152和microRNA-602检测在肝癌诊断及手术疗效评估中的应用价值。采用实时荧光定量PCR检测佳木斯市中心医院2012年3月～2015年3月的19例肝癌血清标本中microRNA-152和microRNA-602的表达水平,分析microRNA-152和microRNA-602在乙型肝炎病毒(HBV)阳性组、HBV阴性组和健康对照组血清样本中的表达差异。结果发现 HBV阳性血清样本中microRNA-152的表达水平(0.65±0.29)明显低于健康组(1.21±0.32),microRNA-602 在HBV阳性血清样本中的表达(0.63±0.31)明显高于健康组(0.44±0.15),且水平表达的差异具有统计学意义(p＜0.05)。可见血清样本中的microRNA-152和microRNA-602可以用于HBV阳性肝癌诊断的血清标记物,microRNA-152可以用于HBV阳性肝癌术后效果评价的血清标记物。 |
| 关键词: HBV 肝癌 MicroRNA-152 MicroRNA-602 |
| DOI：10.3969/j.issn.1672-5565.2015.03.08 |
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| 基金项目: |
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| Study on the expression and diagnostic significance of microRNA-152 and microRNA-602 in hepatocellular carcinoma |
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ZHOU Yibing
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(Jiamusi Center Hospital General Surgery,Heilongjiang Jiamusi 154002,China)
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| Abstract: |
| To explore the application value of microRNA-152 and microRNA-602in the diagnosis of liver cancer and evaluation of therapeutic effects. The serum microRNA was extracted, and the levels of circulating microRNA-152 and microRNA-602 were quantified by real-time quantitative RT-PCR. Expression differences of microRNA-152 and microRNA-602 were analyzed in every experimental groups. Results show the expression level of the HBV positive hepatocellular carcinoma microRNA-152 was significantly lower than that in the healthy group(p<0.05), while the expression level of microRNA-602 was higher than that in the healthy group(p<0.05). It is speculated that microRNA-152 and microRNA-602 may be employed as novel serum markers for the diagnosis of HBV-positive liver cancer and microRNA-152 may be employed as novel serum markers for the evaluation of therapeutic effect. |
| Key words: Hepatitis B Virus Hepatocellular Carcinoma MicroRNA-152 MicroRNA-602 |
