| 摘要: |
| 搜集与偏头痛相关的编码酶的基因,利用KEGG通路分析目标基因的分布和功能,促进偏头痛遗传学研究和新药靶点研究。以“gene name” AND migraine检索PUBMED数据库,从原始文献中搜集并整理偏头痛相关酶基因数据,用DAVID在线分析工具对数据进行处理。搜索得到31个偏头痛酶基因,对7条KEGG代谢通路进行了分析:色氨酸代谢通路、酪氨酸代谢通路、精氨酸和脯氨酸代谢通路、叶酸一碳单位循环代谢通路、药物代谢通路、外源物质细胞色素P450代谢通路、肾素血管紧张素代谢通路。其中药物代谢通路包括9个药物,又以高选择性5-羟色胺重摄取抑制剂西酞普兰的应用前景最大。DDC、DBH、MTHFD1等6个偏头痛相关基因需要完善多态性研究。CYP450和单胺氧化酶在偏头痛的病理和治疗中都占有重要的地位。通过分析疾病相关酶基因的代谢通路,有助于了解疾病的分子病理基础,并为新药设计提供可靠靶点。 |
| 关键词: 偏头痛基因 酶 KEGG pathway DAVID 通路分析 |
| DOI：10.3969/j.issn.1672-5565.2014.03.11 |
| 分类号:Q39 |
| 基金项目: |
|
| Migraine associated enzymes and KEGG pathway analysis |
|
HUANG Rui, ZHENG Heng
|
|
(School of life science and technology,China Pharmaceutical University, Nanjing 210009, China)
|
| Abstract: |
| We collected genes encoding enzymes associated with migraine susceptibility, and did KEGG pathway analysis, to serve for migraine drug designing and genetic studying.First,we searched Pubmed using keywords “gene name” AND migraine to collect target genes from original literatures, then we used an online tool called DAVID for KEGG pathway analysis. Thirty-one genes were finally collected. Seven pathway lists were taken into analysis : Tryptophan metabolism, Tyrosine metabolism, Arginine and proline metabolism, One carbon pool by folate, Drug metabolism, Metabolism of xenobiotics by cytochrome P450, Renin-angiotensin system. Six genes (DDC, DBH, MTHFD1, TYMS, GSTM, REN) were claimed for further polymorphism research through our analysis. One drug Citalopram revealed a rosy prospect in migraine treatment as a high selective serotonin reuptake inhibitor. We also concluded that CYP450 and MAOA played important roles in migraine pathophysiology and treatment. Through pathway analysis of disease associated genes/enzymes,we claimed that it can help to understand the molecular basis of diseases and provide potential targets for drug development. |
| Key words: Migraine gene Enzyme KEGG pathway DAVID Pathway analysis |
