| 引用本文: | 苏炜华,张玉叶,黄宁,肖新换,黄珑,罗俊,阙友雄.甘蔗细胞色素P450还原酶基因的电子克隆与分析[J].生物信息学,2014,12(02):99-105. |
| SU Weihua,ZHANG Yuye,HUANG Ning,XIAO Xinhuan,HUANG Long,LUO Jun,QUE Youxiong.Electronic cloning and characterization of sugarcane ScCYP450 gene using bioinformatics tools[J].Chinese Journal of Bioinformatics,2014,12(02):99-105. |
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| 甘蔗细胞色素P450还原酶基因的电子克隆与分析 |
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苏炜华,张玉叶,黄宁,肖新换,黄珑,罗俊,阙友雄
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( 福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室/国家甘蔗产业技术研发中心,福建 福州 350002 )
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| 摘要: |
| 旨在为甘蔗细胞色素P450还原酶基因的实验克隆、功能鉴定及其应用提供参考。本研究以甘蔗类似细胞色素P450还原酶基因的EST序列CF576130.1为探针,在甘蔗EST数据库中进行检索,并基于电子克隆技术获得了甘蔗细胞色素P450还原酶基因(Cytochrome P450 reductase)的一条cDNA全长序列,命名为ScCYP450。采用生物信息学方法,对该基因编码蛋白从氨基酸组成、理化性质、亚细胞定位、跨膜结构域、疏水性/亲水性、高级结构及功能域等方面进行预测和分析。结果表明该基因全长1 821 bp,包含一个744 bp的开放阅读框,编码247个氨基酸,该基因编码蛋白定位于细胞内质网(膜),为可溶性碱性蛋白,不存在信号肽,二级结构原件多为无规卷曲,含有多个保守功能域,主要功能为辅酶因子生物合成和翻译功能。 |
| 关键词: 甘蔗 ScCYP450基因 电子克隆 生物信息学 |
| DOI：10.3969/j.issn.1672-5565.2014-02.20140204 |
| 分类号:TS242.1;Q753 |
| 基金项目:国家自然科学基金(31340060)；农业部948项目(2014-S18)；教育部博士点基金(20103515120006)；福建农林大学杰出青年基金(xjq201202)资助。 |
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| Electronic cloning and characterization of sugarcane ScCYP450 gene using bioinformatics tools |
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SU Weihua, ZHANG Yuye, HUANG Ning, XIAO Xinhuan, HUANG Long, LUO Jun, QUE Youxiong
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(Key Laboratory of Sugarcane Biology and Genetic Breeding,Ministry of Agriculture,Fujian Agriculture and Forestry University/Sugarcane Research & Development Center,China Agriculture Research System,Fuzhou 350002,China )
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| Abstract: |
| The present study aims to provide references for research on the experimental cloning, function identification and its application of sugarcane Cytochrome P450 reductase gene. The full-length cDNA sequence of one sugarcane Cytochrome P450 reductase gene termed ScCYP450, was obtained by in silico cloning using CF576130.1 sequence from sugarcane as the probe sequence. Using bioinformatics methods, several characteristics of ScCYP450 encoding amino acids, including the amino acid composition, physical and chemical properties, the subcellular localization, the transmembrane domain structure, hydrophobic/hydrophilic, advanced structure and forecast and plus the functional domains, were analyzed. The result showed that this gene is 1 821 bp length and contains a 744 bp open reading frame, encoding 247 amino acids, and the encoded protein localizes in cells endoplasmic reticulum (film), as the alkaline soluble protein. There is no signal peptide. The secondary structure mostly consistes of random coil. ScCYP450 protein contains several conservative functional domains, and the main function of this protein involved in growth factor biosynthesis and transcription. |
| Key words: Sugarcane ScCYP450 gene In silico cloning Bioinformatics |
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