| 引用本文: | 许朋,牛宪立,姬可平.贵州桐梓何首乌rDNA ITS序列分析[J].生物信息学,2014,12(1):27-32. |
| XU Peng,NIU Xianli,JI Keping.Analysis of rDNA ITS sequences of polygonum multiflorum Thunb from Tongzi of Guizhou Province[J].Chinese Journal of Bioinformatics,2014,12(1):27-32. |
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| 摘要: |
| 以改进的CTAB法对何首乌总基因组DNA进行提取,采用通用引物对不同来源的何首乌rDNA ITS序列进行PCR扩增、测序和序列分析。结果表明,何首乌rDNA完全序列片段长度共约652 bp,其中ITS1的长度为202 bp,5.8 S的长度为161 bp,ITS2长度为232 bp,与其近缘种ITS序列间存在明显差异。其rDNA ITS序列在分子水平上为鉴别何首乌提供了参考依据。 |
| 关键词: 何首乌 ITS序列 PCR |
| DOI：10.3969/j.issn.1672-5565.2014-01.20140105 |
| 分类号:R978.1+6 |
| 基金项目: |
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| Analysis of rDNA ITS sequences of polygonum multiflorum Thunb from Tongzi of Guizhou Province |
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XU Peng,NIU Xianli,JI Keping
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(Zhuhai Campus of Zunyi Medical collage,Guangdong Zhuhai 519041,China)
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| Abstract: |
| With improved CTAB method, Polygonum total genomic DNA was extracted using universal primers for different sources of Polygonum rDNA ITS sequences for PCR amplification, sequencing and sequence analysis. The results showed that Polygonum rDNA sequence is a 652 bp fragment, in which the lengths of ITS1,5.8 S and ITS2 are 202 bp, 161 bp, and 232 bp, respectively. The ITS sequences indicate that Polygonum has significant difference from its related species . The rDNA ITS sequences at the molecular level can provide a reference for the identification of Polygonum. |
| Key words: Polygonum multiflorum ITS sequence PCR |
