| 引用本文: | 王亚芳,薛良义.大黄鱼白细胞介素8基因克隆及特征分析[J].生物信息学,2013,11(3):233-236,242. |
| WANG Ya-fang,XUE Liang-yi.Cloning and Characterization Analysis of Interleukin-8Gene in Larimichthys crocea[J].Chinese Journal of Bioinformatics,2013,11(3):233-236,242. |
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| 摘要: |
| 白细胞介素8是一种CXC 型趋化性细胞因子,在免疫反应中起着非常重要的作用。本文在构建大黄鱼肌肉组织cDNA文库的基础上,克隆了白细胞介素8基因。克隆到的白细胞介素8全长为2582bp,基因组包含106bp 的5′端非编码区,52bp的外显子I,168bp的内含子I,133bp的外显子II,149bp的内含子II,87bp的外显子Ⅲ,682bp的内含子Ⅲ,13bp的外显子IV和1192bp 的3′端非编码区,编码序列285bp,编码94个氨基酸。氨基酸序列具有趋化性因子CXC家族的结构特征,在进化上高度保守,与鲈鱼的同源性在90%以上。在检测的大黄鱼的10种组织中,表达量较高的为肾、肝、肠和脾,脑、心和肌肉中表达量较低。 |
| 关键词: 大黄鱼 白细胞介素8 基因结构 组织表达 |
| DOI：10.3969/j.issn.1672-5565.2013-03.20130312 |
| 分类号: |
| 基金项目:国家自然科学基金(30871916)和宁波大学创新性开放实验项目建设专项经费资助(Cxxkf-201221)。 |
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| Cloning and Characterization Analysis of Interleukin-8Gene in Larimichthys crocea |
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WANG Ya-fang , XUE Liang-yi
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(College of Marine Sciences, Ningbo University,Ningbo 315211,China)
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| Abstract: |
| Interleukin-8is a CXC chemokine, and plays an important role in immune reaction. On the basis of constructing the full length cDNA library from the skeletal muscle, interleukin-8gene was cloned and sequenced in Larimichthys crocea. The aquired interleukin-8gene is 2582bp in length, including 106bp 5′-UTR, 52bp exonⅠ, 168bp intronⅠ, 133bp exonⅡ, 149bp intronⅡ, 87bp exonⅢ, 682bp intronⅢ, 13bp exon IV, and 1191bp 3′-UTR. The coding region is 285bp which encodes 94amino acids. The deduced amino acid sequence contains CRC domain, and is highly conserved in the evolution. The sequence similarity of Interleukin-8between Larimichthys crocea and Lateolabrax japonicus is over 90%. In the examined ten tissues, interleukin-8gene was expressed highly in kidney, liver, intestinal, and spleen, and low in heart, skeletal muscle and brain. |
| Key words: Larimichthys Crocea Inerleukin-8 Gene Structure Tissue Expression |
