| 引用本文: | 仇 键,刘实忠,张志平,魏 芳,杨文凤,罗世巧,校现周.橡胶草异戊烯焦磷酸异构酶基因的电子克隆及分析[J].生物信息学,2013,11(3):209-215. |
| QIU Jian,LIU Shi-zhong,ZHANG Zhi-ping,WEI Fang,YANG Wen-feng,LOU Shi-qiao,XIAO Xian-zhou.In silicon cloning and bioinformatic analysis of isopentenyl diphosphate isomerase genes in Taraxacum kok-saghyz[J].Chinese Journal of Bioinformatics,2013,11(3):209-215. |
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| 摘要: |
| 利用电子克隆方法获得两条橡胶草异戊烯焦磷酸异构酶基因 ( IDI) 的cDNA序列和编码区基因组序列,分别命名为TkIDI1和TkIDI2,并采用生物信息学方法对该基因及其编码蛋白进行系统进化、亚细胞定位、活性位点、高级结构等方面的预测和分析。结果显示,TkIDI1的cDNA序列长度为980bp,包含一个696bp 的开放读码框 (ORF),编码232个氨基酸,预测定位于内质网或叶绿体上；TkIDI2的cDNA序列长度为1038bp,包含一个843bp的ORF,编码281个氨基酸,预测定位于线粒体或叶绿体；而他们的截短转录本蛋白定位于胞质中,且都具有过氧化物酶体定位信号。结构分析表明TkIDI1和TkIDI2与植物IDI蛋白同源,活性位点保守,高级结构与其他植物的IDI均具有高度的相似性。 |
| 关键词: 橡胶草 异戊烯焦磷酸异构酶 电子克隆 生物信息学分析 |
| DOI：10.3969/j.issn.1672-5565.2013-03.20130309 |
| 分类号: |
| 基金项目:国家天然橡胶产业体系 (CARS-34-GW7) 和中国热带农业科学院橡胶研究所基本科研业务费专项 (1630022011021) 资助。 |
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| In silicon cloning and bioinformatic analysis of isopentenyl diphosphate isomerase genes in Taraxacum kok-saghyz |
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QIU Jian,LIU Shi-zhong,ZHANG Zhi-ping, WEI Fang, YANG Wen-feng,LOU Shi-qiao, XIAO Xian-zhou
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( Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture P. R. China; Rubber Research Institute, CATAS, Danzhou 571737,China)
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| Abstract: |
| Two isopentenyl diphosphate isomerase genes (TkIDI1and TkIDI2) were obtained successfully from Taraxacum kok-saghyz using in silicon cloning technique. Some characters of the IDI gene and encoded protein sequences were predicted and analyzed by the bioinformatics methods in the following aspects, such as phylogenetic tree, signal peptide, localization sites in cells, location of active sites, secondary and tertiary structure. Results showed that TkIDI1(980bp) contains a complete ORF (696bp) encoding 232amino acid and was predicted to locate at endoplasmic reticulum or chloroplast; TkIDI2(1038bp) contains a complete ORF (843bp) encoding 281amino acid and was predicted to locate at mitochondrion or chloroplast; their truncated transcript productions were the cytoplasm located and presented PTS1predicted as peroxisomal targeting. Protein structure prediction suggested that TkIDIs (TkIDI1and TkIDI2) are highly homologous to other IDIs originate from various plants, and exhibit similar location of active sites and protein structure. |
| Key words: Taraxacum Kok-saghyz Isopentenyl Diphosphate Isomerase(IDI) In Silicon Cloning Bioinformatics Analysis |
