| 引用本文: | 徐洪来,肖 敏,杨 超,高 义,雒真龙,周鸿敏,陈忠华.应用 PPIN分析肝移植临床耐受患者PBMC基因表达特点[J].生物信息学,2013,11(2):136-141. |
| XU Hong-lai,XIAO Min2,YANG Chao,GAO Yi,LUO Zhen-long,ZHOU Hong-min,CHEN Zhong-hua.Apply protein-protein interaction network to analyze characteristics of the geneexpression in PBMC of operational tolerant liver transplant recipients[J].Chinese Journal of Bioinformatics,2013,11(2):136-141. |
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| 应用 PPIN分析肝移植临床耐受患者PBMC基因表达特点 |
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徐洪来1,肖 敏2,杨 超1,高 义1,雒真龙1,周鸿敏1,陈忠华1
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(1.华中科技大学同济医学院附属同济医院 器官移植研究所,湖北 武汉430030; ;2.华中科技大学同济医学院附属协和医院 皮肤科,湖北 武汉430022)
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| 摘要: |
| 应用生物信息学方法分析肝移植临床耐受患者PBMC基因表达特征,筛选临床耐受关键基因。从GEO数据库获取19个肝移植临床耐受病例及22个非临床耐受病例基因表达谱数据。应用DAVID网络软件进行差异基因功能注释与聚类分析；通过Cytoscape软件的MiMI插件构建蛋白质相互作用网络(PPIN)筛选肝移植临床耐受关键基因。差异基因涉及蛋白质及RNA代谢、免疫应答、膜结构调节等复杂生物过程。PPIN网络分析获得10个临床耐受核心基因。我们的研究表明:肝移植临床耐受涉及外周血免疫细胞复杂的基因表达调控机制及蛋白质间相互作用；RNA的转录后加工及蛋白质降解在免疫耐受的形成中发挥了重要作用；RBM8A、DHX9、CBL、IKBKB、CSNK2A1、HSPA8等核心基因发挥重要的免疫调节功能。 |
| 关键词: 肝移植 临床耐受 基因表达谱 蛋白质相互作用网络 |
| DOI：10.3969/j.issn.1672-5565.2013-02.20130211 |
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| 基金项目:国家重点基础研究发展计划(973计划)(2009CB522407)。 |
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| Apply protein-protein interaction network to analyze characteristics of the geneexpression in PBMC of operational tolerant liver transplant recipients |
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XU Hong-lai1, XIAO Min2, YANG Chao1, GAO Yi1, LUO Zhen-long1, ZHOU Hong-min1, CHEN Zhong-hua1
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(1.Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan,430030, China; ;2.Department of Dermatology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430022, China)
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| Abstract: |
| In this paper we use methods of bioinformatics to explore the characteristics of the gene expression and screen key genes in PBMCs of operational tolerant patients receiving liver transplantation using bio-informatics. A data-set of gene expression profiles of 19operational tolerant samples of liver transplantation and 22non-tolerant was downloaded from database GEO. We applied DAVID network software to annotate and functionally classified the differential genes. The MiMI plug-in for cytoscape was used to screen key genes related to operational tolerance by protein-protein interaction network(PPIN)analysis. Differential genes were involved in complicated and diversified biological processes such as metabolism of protein and RNA, immune response, membrane organization and so on.10core genes highly related to operational tolerance were obtained by PPIN analysis. Our results indicate that the immune mechanism of operational tolerance of liver grafts involves sophisticated regulation mechanism of gene expression and extensive protein-protein interaction in PBMCs; RNA post-transcriptional processing and proteins degradation are important participants in regulation of operational tolerance; Core genes such as RBM8A, DHX9, CBL, IKBKB, HSPA8etc., play key regulatory roles in immune functions. |
| Key words: Liver Transplantation Operational Tolerance Gene Expression Profile PPIN |
