| 引用本文: | 黄 宁,张玉叶,苏亚春,罗 俊,王建华,苏炜华,许莉萍,阙友雄.甘蔗天冬氨酰半醛脱氢酶基因的电子克隆与分析[J].生物信息学,2013,11(2):91-99. |
| HUANG Ning,ZHANG Yu-ye,SU Ya-chun,LUO Jun,WANG Jian-hua,SU Wei-hua,XU Li-Ping,QUE You-xiong.Electronic cloning and characterization of sugarcane ScASADH gene using bioinformatics tools[J].Chinese Journal of Bioinformatics,2013,11(2):91-99. |
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| 甘蔗天冬氨酰半醛脱氢酶基因的电子克隆与分析 |
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黄 宁, 张玉叶, 苏亚春,罗 俊 ,王建华, 苏炜华, 许莉萍 ,阙友雄
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(福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室/国家甘蔗产业技术研发中心,福建 福州 350002)
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| 摘要: |
| 应用电子克隆技术,以水稻EF576477序列为探针,获得了甘蔗天冬氨酰半醛脱氢酶基因( aspartate-semialdehyde dehydrogenase,ASADH )的一条cDNA全长序列,命名为ScASADH。采用生物信息学方法,对该基因编码蛋白从氨基酸组成、理化性质、亚细胞定位、跨膜结构域、疏水性/亲水性、 高级结构及功能域等方面进行预测和分析。结果表明:该基因全长1711bp,包含一个1128bp的开放阅读框,编码375个氨基酸,该基因编码蛋白定位于细胞核,为可溶性蛋白,存在信号肽,二级结构原件多为无规卷曲,含有多个保守功能域,主要功能为翻译。电子表达分析结果显示,该基因在甘蔗根尖、幼苗、花序、叶片和茎中组成型表达,其中在茎中的表达量比其他组织类型中表达量高。该基因的表达受葡萄糖杆菌和赤腐病菌的调控。 |
| 关键词: 甘蔗 ASADH基因 电子克隆 生物信息学 电子表达分析 |
| DOI：10.3969/j.issn.1672-5565.2013-02.20130203 |
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| 基金项目:国家自然科学基金“甘蔗抗黑穗病分子机理及关键基因克隆和功能研究”(31101196)；现代农业产业技术体系建设专项资金项目“国家甘蔗产业技术体系”(CARS-20)；福建农林大学杰出青年科研人才基金(xjq201202)。 作者简介:黄宁,女,汉族,山东省枣庄市人,在读硕士研究生,E-mail:hning2012@126.com. |
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| Electronic cloning and characterization of sugarcane ScASADH gene using bioinformatics tools |
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HUANG Ning,ZHANG Yu-ye,SU Ya-chun,LUO Jun,WANG Jian-hua, SU Wei-hua,XU Li-Ping,QUE You-xiong
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(Key Laboratory of Sugarcane Biology and Genetic Breeding , Ministry of Agriculture,Fujian Agriculture and Forestry University/Sugarcane Research & Development Center, China Agriculture Research System,Fuzhou 350002, China )
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| Abstract: |
| The full-length cDNA sequence of one sugarcane aspartate-semialdehyde dehydrogenase gene(ScASADH gene)was abtained by in silico cloning using EF576477sequence from Oryza sativa.as the probe sequence. Some characters of the ScASADH gene encoding amino acid, including the composition of amino acid sequence, physical and chemical properties, subcellular localization, transmembrane domain, hydrophobicity/hydrophilicity, secondary and tertiary structure of protein plus functional domains, were analyzed by bioinformatics tools. The results showed that the full-length ScASADH gene from sugarcane was 1711bp, including one 1128bp open reading frame(ORF)which encodes a polypeptide of 375amino acids. The encoded protein of ScASADH gene, with the presence of signal peptide and several conserved domain sequences, was soluble and located in nucleus, and the corresponding secondary structure of this protein was mainly composed of random coil. The function of the ScASADH protein was mainly involved with translation. Electronic expression analysis revealed that the ScASADH gene was constitutively expressed in the sugarcane root tip, seedling, inflorescence, leaf and stem, and the expression of this gene in the stem of sugarcane was much higher than that in all the other four types of sugarcane tissues.the expression of this gene was regulated under the stresses of Gluconacetobacter and Colletotrichum falcatum. |
| Key words: Sugarcane ASADH Gene In Silico Cloning Bioinformatics Electronic Expression Analysis |
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